Facultad de Ciencias de la Tierra y el Mar
URI permanente para esta comunidadhttp://10.0.96.45:4000/handle/11056/14052
La Facultad de Ciencias de la Tierra y Mar (FCTM) de la Universidad Nacional (UNA), se crea en el año 1973 y desde sus inicios se ha caracterizado por su compromiso con los principios y valores que sustentan la Universidad Necesaria; la cual en lo fundamental se caracteriza por la búsqueda de nuevos espacios que le permitan seguir aportando a la sociedad profesionales y productos de la investigación, extensión y servicios que contribuyan a la mejora en la calidad de vida de la población costarricense y sus relaciones con la naturaleza.
Decana: Grace Wong Reyes, Ph.D, correo electrónico: grace.wong.reyes@una.cr
Contacto:
Página web: www.tierraymar.una.ac.cr
Correo: fctm@una.cr
Teléfono: (506) 2277-3226 / (506) 2277-3280
Biblioteca Especializada Facultad de Ciencias de la Tierra y el Mar:
Página web: www.bctm.una.ac.cr
Correo: bctm@una.cr
Teléfono: 2562-6490 / 2562-6491
Facebook: @BibliotecaTierrayMar
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Ítem A case study on pharmaceutical residues and antimicrobial resistance genes in Costa Rican rivers : a possible route of contamination for feline and other species(Elsevier, 2023-11-12) Vargas-Villalobos, Seiling; Hernández, Felix; Fabregat-Safont, David; Salas-González, Denis; Quesada-Alvarado, Francisco; Botero-Coy, Ana Maria; Esperón, Fernando; Martín-Maldonado, Bárbara; Monrós-González, Juan; Ruepert, Clemens; Estrada-König, Sandra; Rivera-Castillo, Josué; Chaverri-Fonseca, Fabio; Blanco-Peña, KinndleIn this investigation, the presence of antibiotics and pharmaceuticals in Costa Rican surface waters, specifically in regions near feline habitats, was examined. The study revealed that 47% of the water samples contained detectable traces of at least one antibiotic. Ciprofloxacin and norfloxacin were the most frequently detected compounds, each with a detection rate of 27%. Other antibiotics, such as erythromycin, roxithromycin, and trimethoprim, were also found but at lower frequencies, around 14%. Notably, all antibiotic concentrations remained below 10 ng/L, with ciprofloxacin, norfloxacin, and erythromycin showing the highest concentrations. Furthermore, the investigation revealed the presence of non-antibiotic pharmaceutical residues in the water samples, typically at concentrations below 64 ng/L. Tramadol was the most frequently detected compound, present in 18% of the samples. The highest concentrations were observed for acetaminophen and tramadol, measuring 64 and 10 ng/L, respectively. Comparing these findings with studies conducted in treated wastewater and urban rivers, it became evident that the concentrations of antibiotics and pharmaceuticals were notably lower in this study. While previous research reported higher values, the limited number of studies conducted in protected areas raises concerns about the potential environmental impact on biodiversity. In summary, these results emphasize the importance of monitoring pharmaceutical residues and antimicrobial resistance genes ARGs in vulnerable ecosystems, especially those in close proximity to feline habitats in Costa Rica. Additionally, the study delved into the detection of (ARGs). All tested water samples were positive for at least one ARG, with the blaTEM gene being the most prevalent at 82%, followed by tetS at 64% and qnrB at 23%. Moreover, this research shed light on the complexity of evaluating ARGs in environmental samples, as their presence does not necessarily indicate their expression. It also highlighted the potential for co-selection and coregulation of ARGs, showcasing the intricate behaviors of these genes in aquatic environments.Ítem A Combined Metagenomics and Metatranscriptomics Approach to Unravel Costa Rican Cocoa Box Fermentation Processes Reveals Yet Unreported Microbial Species and Functionalities(Frontiers Media, 2021-02-16) Verce, Marko; Schoonejans, Jorn; Hernandez Aguirre, Carlos; Luc De Vuyst; Weckx, StefanCocoa fermentation is the first step in the post-harvest processing chain of cocoa and is important for the removal of the cocoa pulp surrounding the beans and the development of flavor and color precursors. In the present study, metagenomic and metatranscriptomic sequencing were applied to Costa Rican cocoa fermentation processes to unravel the microbial diversity and assess the function and transcription of their genes, thereby increasing the knowledge of this spontaneous fermentation process. Among 97 genera found in these fermentation processes, the major ones were Acetobacter, Komagataeibacter, Limosilactobacillus, Liquorilactobacillus, Lactiplantibacillus, Leuconostoc, Paucilactobacillus, Hanseniaspora, and Saccharomyces. The most prominent species were Limosilactobacillus fermentum, Liquorilactobacillus cacaonum, and Lactiplantibacillus plantarum among the LAB, Acetobacter pasteurianus and Acetobacter ghanensis among the AAB, and Hanseniaspora opuntiae and Saccharomyces cerevisiae among the yeasts. Consumption of glucose, fructose, and citric acid, and the production of ethanol, lactic acid, acetic acid, and mannitol were linked to the major species through metagenomic binning and the application of metatranscriptomic sequencing. By using this approach, it was also found that Lacp. plantarum consumed mannitol and oxidized lactic acid, that A. pasteurianus degraded oxalate, and that species such as Cellvibrio sp., Pectobacterium spp., and Paucilactobacillus vaccinostercus could contribute to pectin degradation. The data generated and results presented in this study could enhance the ability to select and develop appropriate starter cultures to steer the cocoa fermentation process toward a desired course.Ítem A comparison of different indirect techniques to evaluate volcanic intact rock strength(Springer, 2009) Del Potro, Rodrigo; Hürlimann, MarcelThe efficiency of different methods that are used to evaluate volcanic intact rock strength is compared that includes Schmidt hammers, point load tests and uniaxial compression tests. The Schmidt hammer records the rebound height of the mass (R) on a linear scale and gives an indication of the strength of the material being tested. The two types of Schmidt hammers (L- and N-type), with different impact energies are used that shows results as the rebound height RLand RN for the L- and N-type Schmidt hammers, respectively. The other method used is point load test that provides strength measurements of irregular fragments of rocks or of rock cores, with all rock dimensions being greater than 50 mm. The last method is uniaxial compression that shows little scatter and clear logarithmic increase of uniaxial compressive strength with increasing unit weight. The studies showed that Schmidt hammers can give results similar in quality to uniaxial compression tests.Ítem A comparison of the reproductive ability of Varroa destructor (Mesostigmata:Varroidae) in worker and drone brood of Africanized honey bees (Apis mellifera)(Springer Science, 2007-09-08) Calderón, Rafael A.; Zamora, Luis G.; Van Veen, Johan W.; Quesada, Mariela V.Colony infestation by the parasitic mite, Varroa destructor is one of the most serious problems for beekeeping worldwide. In order to reproduce varroa females, enter worker or drone brood shortly before the cell is sealed. To test the hypothesis that, due to the preference of mites to invade drone brood to reproduce, a high proportion of the mite reproduction should occur in drone cells, a comparative study of mite reproductive rate in worker and drone brood of Africanized honey bees (AHB) was done for 370 mites. After determining the number, developmental stage and sex of the offspring in worker cells, the foundress female mite was immediately transferred into an uninfested drone cell. Mite fertility in single infested worker and drone brood cells was 76.5 and 79.3%, respectively. There was no difference between the groups (X 2 = 0.78, P = 0.37). However, one of the most significant differences in mite reproduction was the higher percentage of mites producing viable offspring (cells that contain one live adult male and at least one adult female mite) in drone cells (38.1%) compared to worker cells (13.8%) (X 2 = 55.4, P < 0.01). Furthermore, a high level of immature offspring occurred in worker cells and not in drone cells (X 2 = 69, P < 0.01). Although no differences were found in the percentage of non-reproducing mites, more than 74% (n = 85) of the mites that did not reproduce in worker brood, produced offspring when they were transferred to drone brood.Ítem A Creep Event on the Shallow Interface of the Nicoya Peninsula, Costa Rica Seismogenic Zone(American Geophysical Union, 2004) Protti, Marino; González, Victor; Kato, Teruyuki; Iinuma, Takeshi; Miyazaki, Shinichi; Obana, Koichiro; Kaneda, Yoshiyuki; LaFemina, Peter; Dixon, Timothy; Schwartz, SusanSummary of the paper "A Creep Event on the Shallow Interface of the Nicoya Peninsula, Costa Rica Seismogenic Zone"Ítem A long-term record of polythionates in the acid crater-lake of Poás volcano: Changes in the subaqueous input of fumarolic gases(IAVCEI. General Assembly. Pucón-Chile, 2004-01) Martínez, M.; Van Bergen, M. J.; Fernández, E.; Takano, B.; Malavassi, E.; Barboza, V.; Miura, Y.; Van der Laat, R.; Duarte, E.; Valdés, J.; Sáenz, W.Ítem A multidisciplinary approach to analyze the antimicrobial resistance in natural ecosystems(Elsevier, 2024-02-25) Blanco-Pena, Kinndle; Quesada-Alvarado, Francisco; Salas-González, Denis; Chaverri-Fonseca, FabioAntimicrobial Resistance (AMR) poses a global threat to both human health and environmental well-being. Our study delved into Costa Rican wildlife reserves, uncovering a substantial human impact on these ecosystems and underscoring the imperative to pinpoint AMR hotspots. Embracing a One Health perspective, we advocated for a comprehensive landscape analysis that intricately intertwined geographic, climatic, forest, and human factors. This study illuminated the link between laboratory results and observed patterns of antimicrobial use, thereby paving the way for sustainable solutions. Our innovative methodology involved deploying open-ended questions to explore antimicrobial usage across livestock activities, contributing to establishing a comprehensive methodology. Non-invasive sampling in wildlife emerged as a critical aspect, shedding light on areas contaminated by AMR. Feline species, positioned at the apex of the food chain, acted as sentinels for environmental health due to heightened exposure to improperly disposed waste. Regarding laboratory findings, each sample revealed the presence of at least one antimicrobial resistance gene (ARG). Notably, genes encoding resistance to tetracyclines dominated (94.9%), followed by beta-lactams (75.6%), sulfonamides (53.8%), aminoglycosides (51.3%), quinolones (44.9%), phenicols (25.6%), and macrolides (20.5%). Genes encoding polymyxins were not detected. Moreover, 66% of samples carried a multi-resistant microbiome, with 15% exhibiting resistance to three antimicrobial families and 51% to four. The absence of a correlation between forest coverage and ARG presence underscored the profound human impact on wildlife reserves, surpassing previous estimations. This environmental pressure could potentially modify microbiomes and resistomes in unknown ways. As not all antimicrobial families encoding ARGs were utilized by farmers, our next step involved evaluating other human activities to identify the primary sources of contamination. This comprehensive study contributed crucial insights into the intricate dynamics of AMR in natural ecosystems, paving the way for targeted interventions and sustainable coexistence.Ítem A new dagger nematode, Xiphinema tica n. sp. (Nematoda: Longidoridae), from Costa Rica with updating of the polytomous key of Loof and Luc (1990)(Springer, 2017-05-15) Peraza-Padilla, Walter; Cantalapiedra-Navarrete, Carolina; Zamora-Araya, Tatiana; Palomares-Rius, Juan Emilio; Castillo, Pablo; Archidona Yuste, AntonioA new dagger nematode, Xiphinema tica n. sp., is described and illustrated from several populations extracted from soil associated with several crops and wild plants in Costa Rica. The new dagger nematode is characterised by a moderate body size (3276–4240 μm), a rounded lip region, ca 13.5 μm wide, separated from body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a moderately long odontostyle ca 135 μm long, stylet guiding ring located at ca 122 μm from anterior end, vulva almost equatorial (50–54%), well-developed Z-organ, with heavy muscularised wall containing in the most of specimens observed two moderately refractive inclusions variable in shape (from round to star-shaped), with uterine spines and crystalloid bodies; female tail short, dorsally convex-conoid, with rounded end and a small peg, with a c’ ratio ca 0.8, bearing two or three pairs of caudal pores and male absent. The unique and novel uterine differentiation based on the coexistence of a well-developed Z-organ mixed with uterine spines and crystalloid bodies in Xiphinema prompted us to update and include this combination of characters in the polytomous key of Loof and Luc (1990). Integrative diagnosis was completed with molecular data obtained, using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA, partial 18S–rDNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships of this species with other Xiphinema spp. indicated that X. tica n. sp. was monophyletic to the other species from the morphospecies Group 4, Xiphinema oleae.Ítem A new ring nematode, Xenocriconemella costaricense sp. nov., (Nematoda: Criconematidae) from Costa Rica(Universidad Nacional, Costa Rica, 2024-04-20) Peraza-Padilla, Walter; Aráuz Badilla, Jefferson; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan Emilio; Archidona Yuste, Antonio; Castillo, PabloDuring nematode surveys of natural vegetation in forests of La Cima de Copey de Dota, San José, San José province, Costa Rica, a Xenocriconemella species closely resembling X. macrodora and related species was found. Integrative taxonomical approaches demonstrated that it is a new species described herein as X. costaricense sp. nov. The new species is parthenogenetic (only females have been detected) and characterised by a short body (276–404 μm); lip region with two annuli, not offset, not separated from body contour; first lip annulus partially covering the second lip annulus. Stylet thin, very long (113–133 μm) and flexible, occupying 30.5–47.8% of body length. Excretory pore located from one or two annuli anterior to one or two annuli posterior to level of stylet knobs, at 42 (37–45) μm from anterior end. Female genital tract monodelphic, prodelphic, outstretched, and occupying 35–45% of body length, with vagina slightly ventrally curved (14–18 μm long). Anus located 6–11 annuli from the tail terminus. Tail conoid and bluntly rounded terminus, the last 2–3 annuli oriented dorsally. Results of molecular characterisation and phylogenetic analyses of D2-D3 expansion segments of 28S rRNA, ITS, and partial 18S rRNA, as well as cytochrome oxidase c subunit 1 gene sequences further characterised the new species and clearly separated it from X. macrodora and other related species (X. iberica, X. paraiberica, and X. pradense).Ítem A NEW SPECIES OF NEMATODE, SCLERORHABDITIS NEOTROPICALIS SP.N. (RHABDITIDA), ASSOCIATED WITH AZTECA ANTS IN CECROPIA OBTUSIFOLIA(Nematropica, 2012-06) Esquivel, Alejandro; Abolafia Cobaleda, Joaquín; Hanson, P.; Pinto, A.Sclerorhabditis neotropicalis sp. n. is described and illustrated. It can be distinguished by its body length (515-560 μm in females and 363-455 μm in males), lip region with crown-shaped lips, having acute three acute thorns, oral aperture one third of lip region width, stoma 15-22 μm long or 1.0-1.6 times as long as diameter of lip region, stomatal tube about 1.5 times longer than wide, bearing rhabdia with slightly rugous walls at lumen side, stegostom lacking glottoid apparatus and denticles, pharynx 125-135 μm long in females and 107-131 μm long in males, excretory pore and hemizonid located at 21-22% of the distance from the anterior end. The female reproductive system is didelphicamphidelphic, with ovaries straight or dorsally reflexed, and the vulva is a transverse slit, 55-61% from the anterior end, female tail conoid elongated, 62-76 μm long, having a posterior hyaline portion very slender and with acute tip. The male reproductive system is monorchic, with testis anterio-ventrally reflexed, spicules free, 36-47 μm long or 1.6-1.8 times longer than anal body width, with rounded manubrium, gubernaculum 15-18 μm long or 38-42% of spicule length, and bursa anteriorly open, peloderan, with scalloped margin and bearing eight pairs of genital papillae (1+1/1+1+1+1+2), tail conoid spicate, 23-30 μm long. The new species was found in close association with an ant colony (Azteca constructor Emery, 1876) inside a Cecropia obtusifolia Bertoloni, 1840 tree in a tropical rain forest in Costa Rica. Certain species of Azteca ants live in mutualistic association with Cecropia trees and preliminary evidence suggests that the new nematode species is invariably present in the colonies of at least some of these Azteca species. It is, therefore, possible that the new nematode species represents a third partner in the mutualism, although its role is still unknown.Ítem A New Sulfur and Carbon Degassing Inventory for the Southern Central American Volcanic Arc: The Importance of Accurate Time-Series Data Sets and Possible Tectonic Processes Responsible for Temporal Variations in Arc-Scale Volatile Emissions(AGU Publications, 2017-12-12) Muller, C.; Saballos, A.; Ibarra, M.; LaFemina, Peter; de Moor, Joost Maarten; Kern, Christoph; Avard, Geoffroy; AIUPPA, Alessandro; Protti, Marino; Fischer, TobiasThis work presents a new database of SO2 and CO2 fluxes from the Southern Central American Volcanic Arc (SCAVA) for the period 2015–2016. We report 300 SO2 flux measurements from 10 volcanoes and gas ratios from 11 volcanoes in Costa Rica and Nicaragua representing the most extensive available assessment of this 500 km arc segment. The SO2 flux from SCAVA is estimated at 6,24061,150 T/d, about a factor of three higher than previous estimations (1972–2013). We attribute this increase in part to our more complete assessment of the arc. Another consideration in interpreting the difference is the context of increased volcanic activity, as there were more eruptions in 2015–2016 than in any period since 1980. A potential explanation for increased degassing and volcanic activity is a change in crustal stress regime (from compression to extension, opening volcanic conduits) following two large (Mw>7) earthquakes in the region in 2012. The CO2 flux from the arc is estimated at 22,50064,900 T/d, which is equal to or greater than estimates of C input into the SCAVA subduction zone. Time-series data sets for arc degassing need to be improved in temporal and spatial coverage to robustly constrain volatile budgets and tectonic controls. Arc volatile budgets are strongly influenced by short-lived degassing events and arc systems likely display significant short-term variations in volatile output, calling for expansion of nascent geochemical monitoring networks to achieve spatial and temporal coverage similar to traditional geophysical networks.Ítem A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)(European Journal of Taxonomy, 2017-02-22) Azofeifa Bolaños, José B.; Gigant, L. Rodolphe; Nicolás García, Mayra; Pignal, Marc; Tavares González, Fabiola B.; Hagsater, Eric; Salazar, Gerardo; Reyes Lopez, Delfino; Archila Morales, Fredy Leonel; García-García, José A.; Da Silva, Denis; Allibert, Agathe; Solano Campos, Frank; Rodríguez Jimenes, Guadalupe del Carmen; Paniagua Vásquez, Amelia; Besse, Pascale; Pérez Silva, Araceli; Grisoni, MichelWe describe a new vanilla species growing in sympatry with Vanilla planifolia Jacks. ex Andrews (Orchidaceae) in the province of Limón, Caribbean coast of Costa Rica. The morphology of the reproductive and vegetative organs observed on vines cultivated under shade-house, the nuclear (Internal Transcribed Spacer) and plastid (matK) nucleotide sequences, as well as the contents of aromatic compounds measured in ripe fruits, show that this species is close to but distinct from V. planifolia. The name V. sotoarenasii M.Pignal, Azofeifa-Bolaños & Grisoni sp. nov. is proposed for this new Vanilla species endemic in Costa Rica. It is especially distinguished from V. planifolia by a reduction of about 30% of the size of the fruits and flowers, by a divergence of ITS sequences for at least two species-conserved nucleotides compared to seven other species of the V. planifolia group, and by the presence of anisic compounds and low content of phenolic compounds (including vanillin) in the fruits. These results confirmed the extension of the area of distribution of V. planifolia southward to Costa Rica, where a recent speciation process occurred. Because of its particular agronomic and aromatic properties, V. sotoarenasii sp. nov. could represent a valuable biological resource for the vanilla industry.Ítem A participatory assessment of ecosystem services and human wellbeing in Rural Costa Rica using Photo-Voice(Springer, 2012-03-09) Berbes-Blazquez, MartaHuman well-being is intricately connected to ecosystem services. A keystone contribution to the ecosystem service literature has been the Millennium Ecosystem Assessment, MA, (Ecosystems and human well-being: a framework for assessment, Island Press, Washington, DC; 2003, 2005). Much of the work on ecosystem services to date has focused on the assessment and classification of environmental functions. The need for inclusion of community perspectives in ecosystem assessments has been widely recognized in order to better understand the distribution of impacts and benefits resulting from natural resource use. Communities can offer a direct route to understanding the complex relationships between ecosystems and human well-being and how environmental management affects their livelihoods. Photovoice has been made popular as a tool for participatory needs assessment but it has had limited use in ecosystem assessments to date. The purpose of this paper is twofold: (1) to present the results of a community-level assessment of environmental services in a watershed dominated by pineapple monoculture in Costa Rica; and (2) to evaluate the strengths and the limitations of photovoice as a tool for mapping the relationship between ecosystems and people. I argue that photovoice is an underutilized methodology that has the potential to complement biophysical ecosystem service assessments in the context of impoverished and resource-dependent communities, particularly, since assessing ecosystem services and acting upon that information requires integrating the knowledges of diverse stakeholders, recognizing power imbalances, and grappling with the complexity of social-ecological systems. Processes such as photovoice have the potential to catalyze community self-organization, which is a critical component for empowerment.Ítem A pilot field evaluation on heat stress in sugarcane workers in Costa Rica: What to do next?(Universidad Nacional, Costa Rica, 2009) Van Wendel de Joode, Berendina; Wesseling Hoogervors, Catharina; Crowe, JenniferClimate change is producing major impacts including increasing temperatures in tropical countries, like Costa Rica, where the sugarcane industry employs thousands of workers who are exposed to extreme heat. Objectives: This article outlines a pilot qualitative evaluation of working conditions and heat in the sugarcane industry. Design: A literature review, direct observations and exploratory interviews with workers were conducted to reach a preliminary understanding of the dimensions of heat-related health issues in the sugarcane industry, as a basis for the design of future studies. Results: The industry employs temporary workers from Nicaragua and Costa Rica as well as year-round employees. Temporary employees work 12-hour shifts during the harvest and processing ('zafra') season. In many cases, sugarcane field workers are required to carry their own water and often have no access to shade. Sugar mill workers are exposed to different levels of heat stress depending upon their job tasks, with the most intense heat and workload experienced by the oven ('caldera') cleaners. Conclusions: Research is needed to achieve better understanding of the multiple factors driving and interacting with heat exposures in the sugarcane industry in order to improve the health and safety of workers while maintaining worker productivity. © 2009 Jennifer Crowe et al.Ítem A regression analysis of Q'eqchi' Maya medicinal plants from southern Belize(Universidad Nacional, Costa Rica, 2006) Sánchez Vindas, PabloAmiguet, V.T., Department of Biology, University of Ottawa, P.O. Box 450, Ottawa, Ont. K1N 6N5, Canada; Arnason, J.T., Department of Biology, University of Ottawa, P.O. Box 450, Ottawa, Ont. K1N 6N5, Canada; Maquin, P., Belize Indigenous Training Institute, Punta Gorda, Toledo District, Belize; Cal, V., Belize Indigenous Training Institute, Punta Gorda, Toledo District, Belize; Sänchez-Vindas, P., Herbario Juvenal, Universidad Nacional, Heredia, Costa Rica; Alvarez, L.P., Herbario Juvenal, Universidad Nacional, Heredia, Costa RicaÍtem A scientific note on the use of stingless bees for commercial pollination in enclosures(INRA/DIB/AGIB/EDP Sciences, 2000) Slaa, Ester Judith; Sánchez Chaves, Luis Alejandro; Sandí, Miriam; Salazar, WilliamLas abejas sin aguijón se consideran polinizadores muy importantes en los trópicos, y se sabe que polinizan eficazmente al menos los cultivos. Sin embargo, rara vez se utilizan para la polinización comercial. Hasta donde sabemos, solo se ha publicado un estudio que utiliza abejas sin aguijón para la polinización de cultivos. En ese estudio, Nannotrigona testaceicornis se utilizó con éxito para polinizar fresas en un invernadero en Japón. Hay varias ventajas que pueden hacer que las abejas sin aguijón sean más adecuadas para la polinización de ciertos cultivos que la abeja melífera comúnmente utilizada.Ítem A Selective Ion HPLC-APCI-MS Method for the Quantification of Pentacyclic Triterpenes in an Anxiolytic Botanical Dietary Supplement for the Animal Health Market(Natural Product Incorporation, 2019) Liu, Rui; Carballo Arce, Ana Francis; Singh, Ranpreet; Saleem, Ammar ; Rocha, Marco; Mullally, Martha; Otarola-Rojas, Marco; Poveda Alvarrez, Luis; Sanchez-Vindas, Pablo; Garcia, Mario; Baker, John; Merali, Zul; Guerrero-Analco, José A.; Durst, Tony; Harris, Cory; Arnason, JohnA new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCIMS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3), β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product ZentrolTM. Detections were at low ng on column for 1 and 2 and in low µg range for 3, 4 and 5 using calibration curves within 10-100 ng (R2 > 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify triterpenes of raw materials used in the manufacture of natural health productsÍtem Abordaje de factores de éxito académico desde la unidad académica: El caso de la escuela Ciencias Geográficas, Universidad Nacional, Costa Rica.(Universidad Estatal a Distancia, 2010-05) Araya Ramírez, Iliana; Quirós Arias, Lilliam; Ruiz Hernández, AmaliaLa educación superior enfrenta el reto de responder al cambio social, tecnológico y a las nuevas formas de organización del trabajo. La autoevaluación de carreras universitarias contribuye al mejoramiento continuo de las prácticas de gestión curricular y de la calidad de la educación impartida. La carrera de Ciencias Geográficas con énfasis en Ordenamiento del Territorio de la Universidad Nacional (CCG-UNA) inició un proceso de autoevaluación con miras a la acreditación desde el año 2005, éste ha permitido visibilizar algunos de los factores que contribuyen al éxito académico. El propósito de esta ponencia es identificar los factores de éxito académico que inciden en la graduación, inserción al mercado de trabajo, deserción y rezago.Ítem Abundance, habitat and body measurements of the rare Long-clawed Mouse (Pearsonomys annectens) in the coastal temperate rainforest of southern Chile(Studies on Neotropical Fauna and Environment, 2012) Cádiz, Rodrigo; Figueroa R., Ricardo A.; Figueroa, Rodolfo; Soraya, Corales E.; Murúa, RobertoBased on rodent trappings in eight localities from 1998 to 2005 (14,984 trap-nights), we provide detailed information on abundance, habitat and additional body measurements of the Long-clawed Mouse (Pearsonomys annectens), a rare rodent in the Valdivian temperate rainforest. The Long-clawed Mouse was collected in three localities only in both highly fragmented and continuous forests, including forestry plantations. The sites had relatively mature and closed vegetation and deep soils covered with leaf litter. Males appeared to be larger and heavier than females. Sexually active individuals were found in spring. We conclude that small and geographically restricted populations, low population densities, microhabitat specialization, and dependence on southern temperate forest make long-clawed mice vulnerable to the disappearance of the forest.Ítem Abundancia del jaguar (Panthera onca), otros felinos y sus presas potenciales en el Parque Nacional Santa Rosa, Costa Rica / Abundance of jaguars (Panthera onca), other felids and their potential prey in Santa Rosa National Park, Costa Rica(Universidad Estatal a Distancia, Costa Rica, 2015) Montalvo Guadamuz, Victor; Sáenz Bolaños, Carolina; Ramírez Carvajal, Shirley; Carrillo Jiménez, EduardoAbundance of jaguars (Panthera onca), other felids and their potential prey in Santa Rosa National Park, Costa Rica. Santa Rosa National Park, Guanacaste Province, protects one of the largest remnants of dry forest in Mesoamerica. It is considered an example of how to regenerate forest in an area that for many years was used for cattle. A proper management requires knowledge of trends in mammal populations. We chose species that demand high ecologic integrity. the jaguar (Panthera onca ) and other felines, and their potential prey. Using trace count we assessed abundance on seven trails systematically sampled in 2001 and later in 2011. In 2011 we found more traces of jaguars, less of cougars and the same number for ocelots. In the second sampling we found less herbivores and frugivores, and more omnivores. We believe that the trace method is reliable, rapid and cheap.