Facultad de Ciencias de la Tierra y el Mar
URI permanente para esta comunidadhttp://10.0.96.45:4000/handle/11056/14052
La Facultad de Ciencias de la Tierra y Mar (FCTM) de la Universidad Nacional (UNA), se crea en el año 1973 y desde sus inicios se ha caracterizado por su compromiso con los principios y valores que sustentan la Universidad Necesaria; la cual en lo fundamental se caracteriza por la búsqueda de nuevos espacios que le permitan seguir aportando a la sociedad profesionales y productos de la investigación, extensión y servicios que contribuyan a la mejora en la calidad de vida de la población costarricense y sus relaciones con la naturaleza.
Decana: Grace Wong Reyes, Ph.D, correo electrónico: grace.wong.reyes@una.cr
Contacto:
Página web: www.tierraymar.una.ac.cr
Correo: fctm@una.cr
Teléfono: (506) 2277-3226 / (506) 2277-3280
Biblioteca Especializada Facultad de Ciencias de la Tierra y el Mar:
Página web: www.bctm.una.ac.cr
Correo: bctm@una.cr
Teléfono: 2562-6490 / 2562-6491
Facebook: @BibliotecaTierrayMar
Instagram: @biblioteca.tierraymar
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Ítem 20 años de monitoreo de cinco especies indicadoras de la salud del Bosque en el Parque Nacional Corcovado, Costa Rica(2013-08-22) Eduardo Carrillo; Joel SáenzCosta Rica es uno de los países más avanzados en América Latina en cuanto a la protección de áreas silvestres y sus recursos naturales. Sin embargo, los datos acerca de si la mayoría de las áreas boscosas costarricenses cumplen con los requisitos mínimos para mantener especies silvestres que requieren áreas extensas para sobrevivir son escasos. Los grandes felinos, como el jaguar y el puma (Puma concolor), sus presas, entre ellas, el chanco de monte (Tayassu pecari), la danta (Tapirus bairdii) y el cabro de monte (Mazama americana) están muy relacionados a los bosques primarios y han desaparecido en la mayor parte del país debido, principalmente, a la destrucción de hábitat y a la presión de cacería. Estas especies pueden ser utilizadas como especies indicadoras para estimar la salud del ecosistema y ayudar a definir rutas de comunicación y límites de corredores biológicos, por ejemplo. Este informe presenta los resultados de los últimos 20 años de monitoreo de cinco especies de mamíferos grandes y medianos en el Parque Nacional Corcovado, PNC. Las especies incluidas en este informe son: el jaguar, el puma, el chancho de monte, el saíno y la danta.Ítem 3. Informe Técnico ISA 04 Colaboración Programa ISA Y Asistentes Técnicos de Atención Primaria en Salud del Área de Salud de Talamanca: Aprendizaje y estrategias de difusión sobre el uso de plaguicidas y la salud humana(Universidad Nacional, Costa Rica, 2014-01) Quesada, Rosario; Romero Boniche, Jenny Raquel; van Wendel de Joode, BernaIn the context of the Infants and Environmental Health Program (ISA - www.isa.una.ac.cr) we started working together with the Primary Care Technical Assistants in Health (ATAPS) in the upper Talamanca area, with the support of the Costa Rican Social Security (CCSS). The collaboration of the ATAPS has allowed us to understand even more the knowledge and population's use of pesticides. The support of the Talamanca Health Area (CCSS) made it possible to implement working meetings with ATAPS in the development of a tool for obtain information on the use, knowledge and experience of the population about pesticides. This instrument was built, validated and piloted by the ATAPS during home visits. Also the development of workshops with the ATAPS, allowed the sharing of information on the types of pesticides, their effects health and research findings that the ISA team develops since 2006 in the area. Through the participation of ATAPS and other social actors in the area (such as the Municipality, women's associations, Biological Corridor, governments among others), it was possible to form a Commission1 (see Annex 01) for attend to the problem of the sky bags that constitute a source of contamination in the communities of the indigenous territory. The ISA Program is very interested in maintaining collaboration with the CCSS and to continue working with ATAPS. The purpose of this document is to report on the main activities carried out with the ATAPS.Ítem A botanical extract of Souroubea sympetala and its active principle, betulinic acid, attenuate the cortisol response to a stressor in rainbow trout, Oncorhynchus mykiss(Elsevier, 2016-09-28) Mullally, Martha; Mimeault, Caroline; Otárola Rojas, Marco Antonio; Sanchez Vindas, Pablo; Garcia, Mario; Poveda Alvarez, Luis; Moon, Thomas W.; Gilmour, Kathleen M.; Trudeau, Vance; Arnason, JohnThe capacity of the traditional anxiolytic plant, Souroubea sympetala, and its bioactive principle, betulinic acid (BA), to lower cortisol levels in rainbow trout, Oncorhynchus mykiss, was investigated. Effects at the level of the cortisol-producing interrenal cells were investigated using an in vitro head kidney preparation. Head kidney tissue incubated with S. sympetala extract or BA produced significantly less cortisol in response to adrenocorticotropic hormone (ACTH) than cells incubated with ACTH alone; a 50% reduction in the cortisol response was predicted to occur at 43 μg mL− 1 extract or 71 μg mL− 1 BA. To investigate whether effects on interrenal cell cortisol production translated into effects at the whole-animal level, the plasma cortisol response to an acute stressor, confinement in a net, was assessed. Fish fed a commercial trout pellet diet supplemented with 1 mg kg− 1 fish BA for six days exhibited a significantly lower plasma cortisol response to the acute stressor than fish fed the unsupplemented diet. Collectively, these results demonstrate that S. sympetala and BA act on the interrenal cells of the hypothalamic-pituitary-interrenal (HPI) axis in rainbow trout to significantly attenuate the cortisol response to an acute stressor. These findings suggest that BA is of potential use in mitigating effects of exposure to the stressors commonly encountered in an aquaculture setting. Statement of relevance • Souroubea sympetala extract and betulinic acid reduced ACTH-stimulated cortisol production by head kidney tissue in vitro, with little or no evidence of cytotoxicity.• The cortisol response to an acute stressor was attenuated in rainbow trout fed a commercial diet supplemented with betulinic acid.• The cortisol-reducing properties of the S. sympetala extract and betulinic acid may be useful in mitigating effects of stress in aquaculture, particularly since, as natural products, they may raise less public concern with respect to human consumption.Ítem A brittle failure model for long-period seismic events recorded at Turrialba Volcano, Costa Rica(American Geophysical Union, 2015-01-30) Eyre, Thomas; Bean, Christopher; DE BARROS, LOUIS; Martini, Francesca; Lokmer, Ivan; Mora Fernández, Mauricio Manuel; Pacheco, Javier F.; Soto, Gerardo J.A temporary seismic network, consisting of 23 broadband and six short-period stations, wasinstalled in a dense network at Turrialba Volcano, Costa Rica, between 8 March and 4 May 2011. During thistime 513 long-period (LP) events were observed. Due to their pulse-like waveforms, the hypothesis thatthe events are generated by a slow-failure mechanism, based on a recent new model by Bean et al. (2014), istested. A significant number (107) of the LPs are jointly inverted for their source locations and mechanisms,using full-waveform moment tensor inversion. The locations are mostly shallow, with depths < 800 m belowthe active Southwest Crater. The results of the decompositions of the obtained moment tensor solutions showcomplex source mechanisms, composed of high proportions of isotropic and low, but seemingly significant,proportions of compensated linear vector dipole and double-couple components. It is demonstrated that thiscan be explained as mode I tensile fracturing with a strong shear component. The source mechanism is furtherinvestigated by exploring scaling laws within the data. The LPs recorded follow relationships very similar tothose of conventional earthquakes, exhibiting frequency-magnitude and corner frequency versus magnituderelationships that can be explained by brittle failure. All of these observations indicate that a slow-failure sourcemodel can successfully describe the generation of short-duration LP events at Turrialba VolcanoÍtem A Combined Metagenomics and Metatranscriptomics Approach to Unravel Costa Rican Cocoa Box Fermentation Processes Reveals Yet Unreported Microbial Species and Functionalities(Frontiers Media, 2021-02-16) Verce, Marko; Schoonejans, Jorn; Hernandez Aguirre, Carlos; Luc De Vuyst; Weckx, StefanCocoa fermentation is the first step in the post-harvest processing chain of cocoa and is important for the removal of the cocoa pulp surrounding the beans and the development of flavor and color precursors. In the present study, metagenomic and metatranscriptomic sequencing were applied to Costa Rican cocoa fermentation processes to unravel the microbial diversity and assess the function and transcription of their genes, thereby increasing the knowledge of this spontaneous fermentation process. Among 97 genera found in these fermentation processes, the major ones were Acetobacter, Komagataeibacter, Limosilactobacillus, Liquorilactobacillus, Lactiplantibacillus, Leuconostoc, Paucilactobacillus, Hanseniaspora, and Saccharomyces. The most prominent species were Limosilactobacillus fermentum, Liquorilactobacillus cacaonum, and Lactiplantibacillus plantarum among the LAB, Acetobacter pasteurianus and Acetobacter ghanensis among the AAB, and Hanseniaspora opuntiae and Saccharomyces cerevisiae among the yeasts. Consumption of glucose, fructose, and citric acid, and the production of ethanol, lactic acid, acetic acid, and mannitol were linked to the major species through metagenomic binning and the application of metatranscriptomic sequencing. By using this approach, it was also found that Lacp. plantarum consumed mannitol and oxidized lactic acid, that A. pasteurianus degraded oxalate, and that species such as Cellvibrio sp., Pectobacterium spp., and Paucilactobacillus vaccinostercus could contribute to pectin degradation. The data generated and results presented in this study could enhance the ability to select and develop appropriate starter cultures to steer the cocoa fermentation process toward a desired course.Ítem A comparison of different indirect techniques to evaluate volcanic intact rock strength(Springer, 2009) Del Potro, Rodrigo; Hürlimann, MarcelThe efficiency of different methods that are used to evaluate volcanic intact rock strength is compared that includes Schmidt hammers, point load tests and uniaxial compression tests. The Schmidt hammer records the rebound height of the mass (R) on a linear scale and gives an indication of the strength of the material being tested. The two types of Schmidt hammers (L- and N-type), with different impact energies are used that shows results as the rebound height RLand RN for the L- and N-type Schmidt hammers, respectively. The other method used is point load test that provides strength measurements of irregular fragments of rocks or of rock cores, with all rock dimensions being greater than 50 mm. The last method is uniaxial compression that shows little scatter and clear logarithmic increase of uniaxial compressive strength with increasing unit weight. The studies showed that Schmidt hammers can give results similar in quality to uniaxial compression tests.Ítem A comparison of fruit removal in Ficus colubrinae between birds and Ectophylla alba (Chiroptera: Phyllostomidae) in a Costa Rican rain forest(Taylor and Francis Ltd., 2021-02-02) De la Llata Quiroga, Edna; Ruedas, Luis; Mora, José ManuelEctophylla alba almost exclusively feeds on the fruits of Ficus colubrinae therefore significantly contributing to dispersal of its seeds. The objective of this research was to quantify the fruit removal of F. colubrinae by E. alba and compare it with that of birds in a F. colubrinae tree at the La Selva Biological Station, Costa Rica. We established seven quadrats randomly at different heights of the tree. We took photographs of each quadrat, quantified the fruits in each photograph, and compared the number of fruits removed during periods of high and low activity. The on-site persistence time of birds (9:05 min) was higher than that of E. alba (0.32 s). Birds removed more fruits (46) than E. alba (41). Birds defecated on site more times (13.08 feces per day) than E. alba (7.5 feces per day). Thus, E. alba is a more important seed disperser of F. colubrinae than are birds, because it persisted a short time at the feeding site, and consumed food beyond the individual trees’ canopies. Most importantly, by defecating during flight or away from the fruiting tree, E. alba disperse seeds some distance from the F. colubrinae tree, thereby contributes more effectively toward seed dispersal.Ítem A Creep Event on the Shallow Interface of the Nicoya Peninsula, Costa Rica Seismogenic Zone(American Geophysical Union, 2004) Protti, Marino; González, Victor; Kato, Teruyuki; Iinuma, Takeshi; Miyazaki, Shinichi; Obana, Koichiro; Kaneda, Yoshiyuki; LaFemina, Peter; Dixon, Timothy; Schwartz, SusanSummary of the paper "A Creep Event on the Shallow Interface of the Nicoya Peninsula, Costa Rica Seismogenic Zone"Ítem A female of Eudorylaimus sp with two vulvae.(Universidad Nacional, Costa Rica, 2001) Esquivel Hernández, AlejandroA female of Eudorylaimus sp, with two vulvae was found in Tempisque Conservation Area in Costa Rica. Thousands of nematodes have been prepared in Cobb's slides to study nematode diversity in Costa Rica; nevertheless, only one aberrant female has been found. The two vulvae are very close to each other and vaginal sclerotization plates show abnormal formation. There is no linkage between the uterus and the gonoducts and the ovaries are poorly developed. An absence of sperm and eggs suggests that the genital apparatus is not functional. Because identification at the species level teas not achieved, more specimens and studies are neededÍtem A long-term record of polythionates in the acid crater-lake of Poás volcano: Changes in the subaqueous input of fumarolic gases(IAVCEI. General Assembly. Pucón-Chile, 2004-01) Martínez, M.; Van Bergen, M. J.; Fernández, E.; Takano, B.; Malavassi, E.; Barboza, V.; Miura, Y.; Van der Laat, R.; Duarte, E.; Valdés, J.; Sáenz, W.Ítem A new dagger nematode, Xiphinema tica n. sp. (Nematoda: Longidoridae), from Costa Rica with updating of the polytomous key of Loof and Luc (1990)(Springer, 2017-05-15) Peraza-Padilla, Walter; Cantalapiedra-Navarrete, Carolina; Zamora-Araya, Tatiana; Palomares-Rius, Juan Emilio; Castillo, Pablo; Archidona Yuste, AntonioA new dagger nematode, Xiphinema tica n. sp., is described and illustrated from several populations extracted from soil associated with several crops and wild plants in Costa Rica. The new dagger nematode is characterised by a moderate body size (3276–4240 μm), a rounded lip region, ca 13.5 μm wide, separated from body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a moderately long odontostyle ca 135 μm long, stylet guiding ring located at ca 122 μm from anterior end, vulva almost equatorial (50–54%), well-developed Z-organ, with heavy muscularised wall containing in the most of specimens observed two moderately refractive inclusions variable in shape (from round to star-shaped), with uterine spines and crystalloid bodies; female tail short, dorsally convex-conoid, with rounded end and a small peg, with a c’ ratio ca 0.8, bearing two or three pairs of caudal pores and male absent. The unique and novel uterine differentiation based on the coexistence of a well-developed Z-organ mixed with uterine spines and crystalloid bodies in Xiphinema prompted us to update and include this combination of characters in the polytomous key of Loof and Luc (1990). Integrative diagnosis was completed with molecular data obtained, using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA, partial 18S–rDNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships of this species with other Xiphinema spp. indicated that X. tica n. sp. was monophyletic to the other species from the morphospecies Group 4, Xiphinema oleae.Ítem A new nesting record for the jabiru in Costa Rica(Waterbird Society, 1998) Villarreal Orias, JohnnyI document the first nesting record of theJabiru (Jabiru mycteria) in "Los Guatuzos" forest floodplain, Alajuela province, Costa Rica. The active nest was located in March 1995 at 10°49'23.70"N and 84°47'23.20"W. The nest was 16m high in a 23m high silk-cotton tree (Ceiba pentandra) and contained three chicks. The zone is not protected and active lumbering is underway.Ítem A new ring nematode, Xenocriconemella costaricense sp. nov., (Nematoda: Criconematidae) from Costa Rica(Universidad Nacional, Costa Rica, 2024-04-20) Peraza-Padilla, Walter; Aráuz Badilla, Jefferson; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan Emilio; Archidona Yuste, Antonio; Castillo, PabloDuring nematode surveys of natural vegetation in forests of La Cima de Copey de Dota, San José, San José province, Costa Rica, a Xenocriconemella species closely resembling X. macrodora and related species was found. Integrative taxonomical approaches demonstrated that it is a new species described herein as X. costaricense sp. nov. The new species is parthenogenetic (only females have been detected) and characterised by a short body (276–404 μm); lip region with two annuli, not offset, not separated from body contour; first lip annulus partially covering the second lip annulus. Stylet thin, very long (113–133 μm) and flexible, occupying 30.5–47.8% of body length. Excretory pore located from one or two annuli anterior to one or two annuli posterior to level of stylet knobs, at 42 (37–45) μm from anterior end. Female genital tract monodelphic, prodelphic, outstretched, and occupying 35–45% of body length, with vagina slightly ventrally curved (14–18 μm long). Anus located 6–11 annuli from the tail terminus. Tail conoid and bluntly rounded terminus, the last 2–3 annuli oriented dorsally. Results of molecular characterisation and phylogenetic analyses of D2-D3 expansion segments of 28S rRNA, ITS, and partial 18S rRNA, as well as cytochrome oxidase c subunit 1 gene sequences further characterised the new species and clearly separated it from X. macrodora and other related species (X. iberica, X. paraiberica, and X. pradense).Ítem A NEW SPECIES OF NEMATODE, SCLERORHABDITIS NEOTROPICALIS SP.N. (RHABDITIDA), ASSOCIATED WITH AZTECA ANTS IN CECROPIA OBTUSIFOLIA(Nematropica, 2012-06) Esquivel, Alejandro; Abolafia Cobaleda, Joaquín; Hanson, P.; Pinto, A.Sclerorhabditis neotropicalis sp. n. is described and illustrated. It can be distinguished by its body length (515-560 μm in females and 363-455 μm in males), lip region with crown-shaped lips, having acute three acute thorns, oral aperture one third of lip region width, stoma 15-22 μm long or 1.0-1.6 times as long as diameter of lip region, stomatal tube about 1.5 times longer than wide, bearing rhabdia with slightly rugous walls at lumen side, stegostom lacking glottoid apparatus and denticles, pharynx 125-135 μm long in females and 107-131 μm long in males, excretory pore and hemizonid located at 21-22% of the distance from the anterior end. The female reproductive system is didelphicamphidelphic, with ovaries straight or dorsally reflexed, and the vulva is a transverse slit, 55-61% from the anterior end, female tail conoid elongated, 62-76 μm long, having a posterior hyaline portion very slender and with acute tip. The male reproductive system is monorchic, with testis anterio-ventrally reflexed, spicules free, 36-47 μm long or 1.6-1.8 times longer than anal body width, with rounded manubrium, gubernaculum 15-18 μm long or 38-42% of spicule length, and bursa anteriorly open, peloderan, with scalloped margin and bearing eight pairs of genital papillae (1+1/1+1+1+1+2), tail conoid spicate, 23-30 μm long. The new species was found in close association with an ant colony (Azteca constructor Emery, 1876) inside a Cecropia obtusifolia Bertoloni, 1840 tree in a tropical rain forest in Costa Rica. Certain species of Azteca ants live in mutualistic association with Cecropia trees and preliminary evidence suggests that the new nematode species is invariably present in the colonies of at least some of these Azteca species. It is, therefore, possible that the new nematode species represents a third partner in the mutualism, although its role is still unknown.Ítem A New Sulfur and Carbon Degassing Inventory for the Southern Central American Volcanic Arc: The Importance of Accurate Time-Series Data Sets and Possible Tectonic Processes Responsible for Temporal Variations in Arc-Scale Volatile Emissions(AGU Publications, 2017-12-12) Muller, C.; Saballos, A.; Ibarra, M.; LaFemina, Peter; de Moor, Joost Maarten; Kern, Christoph; Avard, Geoffroy; AIUPPA, Alessandro; Protti, Marino; Fischer, TobiasThis work presents a new database of SO2 and CO2 fluxes from the Southern Central American Volcanic Arc (SCAVA) for the period 2015–2016. We report 300 SO2 flux measurements from 10 volcanoes and gas ratios from 11 volcanoes in Costa Rica and Nicaragua representing the most extensive available assessment of this 500 km arc segment. The SO2 flux from SCAVA is estimated at 6,24061,150 T/d, about a factor of three higher than previous estimations (1972–2013). We attribute this increase in part to our more complete assessment of the arc. Another consideration in interpreting the difference is the context of increased volcanic activity, as there were more eruptions in 2015–2016 than in any period since 1980. A potential explanation for increased degassing and volcanic activity is a change in crustal stress regime (from compression to extension, opening volcanic conduits) following two large (Mw>7) earthquakes in the region in 2012. The CO2 flux from the arc is estimated at 22,50064,900 T/d, which is equal to or greater than estimates of C input into the SCAVA subduction zone. Time-series data sets for arc degassing need to be improved in temporal and spatial coverage to robustly constrain volatile budgets and tectonic controls. Arc volatile budgets are strongly influenced by short-lived degassing events and arc systems likely display significant short-term variations in volatile output, calling for expansion of nascent geochemical monitoring networks to achieve spatial and temporal coverage similar to traditional geophysical networks.Ítem A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)(European Journal of Taxonomy, 2017-02-22) Azofeifa Bolaños, José B.; Gigant, L. Rodolphe; Nicolás García, Mayra; Pignal, Marc; Tavares González, Fabiola B.; Hagsater, Eric; Salazar, Gerardo; Reyes Lopez, Delfino; Archila Morales, Fredy Leonel; García-García, José A.; Da Silva, Denis; Allibert, Agathe; Solano Campos, Frank; Rodríguez Jimenes, Guadalupe del Carmen; Paniagua Vásquez, Amelia; Besse, Pascale; Pérez Silva, Araceli; Grisoni, MichelWe describe a new vanilla species growing in sympatry with Vanilla planifolia Jacks. ex Andrews (Orchidaceae) in the province of Limón, Caribbean coast of Costa Rica. The morphology of the reproductive and vegetative organs observed on vines cultivated under shade-house, the nuclear (Internal Transcribed Spacer) and plastid (matK) nucleotide sequences, as well as the contents of aromatic compounds measured in ripe fruits, show that this species is close to but distinct from V. planifolia. The name V. sotoarenasii M.Pignal, Azofeifa-Bolaños & Grisoni sp. nov. is proposed for this new Vanilla species endemic in Costa Rica. It is especially distinguished from V. planifolia by a reduction of about 30% of the size of the fruits and flowers, by a divergence of ITS sequences for at least two species-conserved nucleotides compared to seven other species of the V. planifolia group, and by the presence of anisic compounds and low content of phenolic compounds (including vanillin) in the fruits. These results confirmed the extension of the area of distribution of V. planifolia southward to Costa Rica, where a recent speciation process occurred. Because of its particular agronomic and aromatic properties, V. sotoarenasii sp. nov. could represent a valuable biological resource for the vanilla industry.Ítem A participatory assessment of ecosystem services and human wellbeing in Rural Costa Rica using Photo-Voice(Springer, 2012-03-09) Berbes-Blazquez, MartaHuman well-being is intricately connected to ecosystem services. A keystone contribution to the ecosystem service literature has been the Millennium Ecosystem Assessment, MA, (Ecosystems and human well-being: a framework for assessment, Island Press, Washington, DC; 2003, 2005). Much of the work on ecosystem services to date has focused on the assessment and classification of environmental functions. The need for inclusion of community perspectives in ecosystem assessments has been widely recognized in order to better understand the distribution of impacts and benefits resulting from natural resource use. Communities can offer a direct route to understanding the complex relationships between ecosystems and human well-being and how environmental management affects their livelihoods. Photovoice has been made popular as a tool for participatory needs assessment but it has had limited use in ecosystem assessments to date. The purpose of this paper is twofold: (1) to present the results of a community-level assessment of environmental services in a watershed dominated by pineapple monoculture in Costa Rica; and (2) to evaluate the strengths and the limitations of photovoice as a tool for mapping the relationship between ecosystems and people. I argue that photovoice is an underutilized methodology that has the potential to complement biophysical ecosystem service assessments in the context of impoverished and resource-dependent communities, particularly, since assessing ecosystem services and acting upon that information requires integrating the knowledges of diverse stakeholders, recognizing power imbalances, and grappling with the complexity of social-ecological systems. Processes such as photovoice have the potential to catalyze community self-organization, which is a critical component for empowerment.Ítem A pilot field evaluation on heat stress in sugarcane workers in Costa Rica: What to do next?(Universidad Nacional, Costa Rica, 2009) Van Wendel de Joode, Berendina; Wesseling Hoogervors, Catharina; Crowe, JenniferClimate change is producing major impacts including increasing temperatures in tropical countries, like Costa Rica, where the sugarcane industry employs thousands of workers who are exposed to extreme heat. Objectives: This article outlines a pilot qualitative evaluation of working conditions and heat in the sugarcane industry. Design: A literature review, direct observations and exploratory interviews with workers were conducted to reach a preliminary understanding of the dimensions of heat-related health issues in the sugarcane industry, as a basis for the design of future studies. Results: The industry employs temporary workers from Nicaragua and Costa Rica as well as year-round employees. Temporary employees work 12-hour shifts during the harvest and processing ('zafra') season. In many cases, sugarcane field workers are required to carry their own water and often have no access to shade. Sugar mill workers are exposed to different levels of heat stress depending upon their job tasks, with the most intense heat and workload experienced by the oven ('caldera') cleaners. Conclusions: Research is needed to achieve better understanding of the multiple factors driving and interacting with heat exposures in the sugarcane industry in order to improve the health and safety of workers while maintaining worker productivity. © 2009 Jennifer Crowe et al.Ítem A regression analysis of Q'eqchi' Maya medicinal plants from southern Belize(Universidad Nacional, Costa Rica, 2006) Sánchez Vindas, PabloAmiguet, V.T., Department of Biology, University of Ottawa, P.O. Box 450, Ottawa, Ont. K1N 6N5, Canada; Arnason, J.T., Department of Biology, University of Ottawa, P.O. Box 450, Ottawa, Ont. K1N 6N5, Canada; Maquin, P., Belize Indigenous Training Institute, Punta Gorda, Toledo District, Belize; Cal, V., Belize Indigenous Training Institute, Punta Gorda, Toledo District, Belize; Sänchez-Vindas, P., Herbario Juvenal, Universidad Nacional, Heredia, Costa Rica; Alvarez, L.P., Herbario Juvenal, Universidad Nacional, Heredia, Costa RicaÍtem A Selective Ion HPLC-APCI-MS Method for the Quantification of Pentacyclic Triterpenes in an Anxiolytic Botanical Dietary Supplement for the Animal Health Market(Natural Product Incorporation, 2019) Liu, Rui; Carballo Arce, Ana Francis; Singh, Ranpreet; Saleem, Ammar ; Rocha, Marco; Mullally, Martha; Otarola-Rojas, Marco; Poveda Alvarrez, Luis; Sanchez-Vindas, Pablo; Garcia, Mario; Baker, John; Merali, Zul; Guerrero-Analco, José A.; Durst, Tony; Harris, Cory; Arnason, JohnA new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCIMS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3), β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product ZentrolTM. Detections were at low ng on column for 1 and 2 and in low µg range for 3, 4 and 5 using calibration curves within 10-100 ng (R2 > 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify triterpenes of raw materials used in the manufacture of natural health products