Now showing items 1-20 of 176

    • In vivo proteolytic degradation of the Escherichia coli acyltransferase HlyC 

      Guzman-Verri, Caterina; Chaves-Olarte, Esteban; García, Fernando; Arvidson, Staffan; Moreno, Edgardo (The American Society for Biochemistry and Molecular Biology, 2001-05-18)
      Escherichia coli hemolysin (HlyA) is the prototype toxin of a major family of exoproteins produced by Gram-negative bacteria known as “repeats in toxins.” Only fatty acid-acylated HlyA molecules at residues Lys564 and ...
    • GTPases of the Rho subfamily are required for Brucella abortus internalization in nonprofessional phagocytes 

      Guzman-Verri, Caterina; Chaves-Olarte, Esteban; von Eichel-Streiber, Christoph; López-Goñi, Ignacio; Thelestam, Mónica; Arvidson, Staffan; Gorvel, Jean-Pierre; Moreno, Edgardo (The American Society for Biochemistry and Molecular Biology, Inc., 2001-11-30)
      Members of the genus Brucella are intracellular -Proteobacteria responsible for brucellosis, a chronic disease of humans and animals. Little is known about Brucella virulence mechanisms, but the abilities of these ...
    • The two-component system BvrR BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins with counterparts in members of the Rhizobiaceae 

      Guzman-Verri, Caterina; Manterola, Lorea; Sola-Landa, Alberto; Parra, A.; Cloeckaert, A.; GARIN, Jérôme; Gorvel, Jean-Pierre; Moriyon, Ignacio; Moreno, Edgardo; López-Goñi, Ignacio (Proceedings of the National Academy of Sciences, 2002-09-17)
      The Brucella BvrR/BvrS two-component regulatory system is homologous to the ChvI/ChvG systems of Sinorhizobium meliloti and Agrobacterium tumefaciens necessary for endosymbiosis and pathogenicity in plants. BvrR/BvrS ...
    • Activation of Rho and Rab GTPases dissociates Brucella abortus internalization from intracellular trafficking 

      Chaves-Olarte, Esteban; Guzman-Verri, Caterina; Méresse, Stephane; Desjardins, Michel; Pizarro-Cerdá, Javier; Badilla, Jenny; Gorvel, Jean-Pierre; Moreno, Edgardo (Blackwell Science, 2002-10-04)
      Brucella abortus is an intracellular pathogen that relies on unconventional virulence factors to infect hosts. In non-professional phagocytes, Rho GTPasesactivation by the Escherichia coli cytotoxic necrotiz ing factor ...
    • Regulation of Brucella virulence by the two-component system BvrR/BvrS 

      López-Goñi, Ignacio; Guzman-Verri, Caterina; Manterola, Lorea; Sola-Landa, Alberto; Moriyon, Ignacio; Moreno, Edgardo (Elsevier B.V., 2002-12-20)
      The Brucella BvrR/BvrS two-component regulatory system is highly similar to the regulatory and sensory proteins of Sinorhizobium and Agrobacterium necessary for endosymbiosis and pathogenicity in plants, and very similar ...
    • R-Ras Glucosylation and Transient RhoA Activation Determine the Cytopathic Effect Produced by Toxin B Variants from Toxin A-negative Strains of Clostridium difficile 

      Chaves-Olarte, Esteban; Freer, Enrique; Parra, Andrea; Guzman-Verri, Caterina; Thelestam, Monica; Moreno, Edgardo (Universidad Nacional, Costa Rica, 2003-03)
      Clostridium difficile induces antibiotic-associated di-arrhea through the production of toxin A and toxin B;the former toxin has been assumed to be responsible forthe symptoms of the disease. Several toxin ...
    • R-Ras Glucosylation and transient RhoA activation determine the cytopathic effect produced by Toxin B variants from Toxin A-negative strains of clostridium difficile 

      Chaves-Olarte, Esteban; Freer, Enrique; Parra, Andrea; Guzman-Verri, Caterina; Moreno, Edgardo; Thelestam, Mónica (American Society for Biochemistry and Molecular Biology, 2003-03-07)
      Clostridium difficile induces antibiotic-associated diarrhea through the production of toxin A and toxin B; the former toxin has been assumed to be responsible for the symptoms of the disease. Several toxin A-negative ...
    • The effect of the dam–calf relationship on serostatus to Neospora caninum on 20 Costa Rican dairy farms 

      Romero-Zúñiga, Juan José; Frankena, K. (Universidad Nacional, Costa Rica, 2003-03-31)
      An epidemiological study was conduced on 20 dairy herds previously diagnosed as seropositive for Neospora caninum. The number of females per farm varies from 41 to 296. All females present on the farms were bled once in ...
    • Pulmonary sarcocystosis in a puppy with canine distemper in Costa Rica 

      Berrocal, Alexis; López, Alfonso (Universidad Nacional, Costa Rica, 2003-05-01)
      Canine distemper and pulmonary sarcocystosis were diagnosed in a 10-week-old Rottweiler with 4-day history of diarrhea, vomiting, and weakness. Microscopic examination of the lung revealed bronchointerstitial pneumonia ...
    • Vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host 

      Martinez, Isidoro; Jiménez, Carlos; Pauszek, Steven J.; Wertz, Gail W.; Rodriguez, Luis L. (American Society for Microbiology., 2003-07)
      There are two major serotypes of vesicular stomatitis virus (VSV), Indiana (VSIV) and New Jersey (VSNJV).We recovered recombinant VSIVs from engineered cDNAs that contained either (i) one copy of the VSIV G gene(VSIV-GI); ...
    • Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection 

      BARQUERO-CALVO, ELIAS; Chaves-Olarte, Esteban; Weiss, David; Guzman-Verri, Caterina; Chacón-Díaz, Carlos; Rucavado, Alexandra; Moriyon, Ignacio; Moreno, Edgardo (PLOS ONE, 2007-07-18)
      Background. To unravel the strategy by which Brucella abortus establishes chronic infections, we explored its early interaction with innate immunity. Methodology/Principal Findings. Brucella did not induce proinflammatory ...
    • BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence 

      Manterola, Lorea; Guzman-Verri, Caterina; Chaves-Olarte, Esteban; BARQUERO-CALVO, ELIAS; de Miguel, María-Jesús; Moriyon, Ignacio; Grillo, María-Jesús; López-Goñi, Ignacio; Moreno, Edgardo (American Society for Microbiology, 2007-07-30)
      The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption of bvrS or bvrR generates avirulent mutants with altered ...
    • Neurobrucellosis in stranded dolphins, Costa Rica 

      Hernández-Mora, Gabriela; González-Barrientos, Rocio; Morales, Juan Alberto; Chaves-Olarte, Esteban; Guzman-Verri, Caterina; BARQUERO-CALVO, ELIAS; De-Miguel, María-Jesús; Marín, Clara-María; Blasco, Jose-María; Moreno, Edgardo (Universidad Nacional, Costa Rica, 2008-09)
      Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacifi c coast, had meningoencephalitis and antibodies against Brucella spp. Brucella ceti was isolated from cerebrospinal fl uid of 6 dolphins ...
    • Intracellular adaptation of Brucella abortus 

      Lamontagne, Julie; Forest, Anik; Marazzo, Elena; François, Denis; Butler, Heather; Michaud, Jean-François; Boucher, Lyne; Pedro, Ida; Villeneuve, Annie; Sitnikov, Dmitri; Trudel, Sylvie; Nassif, Najib; Boudjelt, Djamila; Tomaki, Fadi; Chaves-Olarte, Esteban; Guzman-Verri, Caterina; Brunet, Sylvain; Côté-Martin, Alexandra; Hunter, Joanna; Moreno, Edgardo; Paramithiotis, Eustache (Universidad Nacional, Costa Rica, 2009-03)
      Macrophages were infected with virulent B. abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared. The virulent strain initially ...
    • Serological diagnosis of brucella infections in odontocetes 

      Hernández-Mora, Gabriela; Manire, Charles A.; González-Barrientos, Rocío; BARQUERO-CALVO, ELIAS; Guzman-Verri, Caterina; Staggs, Lydia; Thompson, Rachel; Chaves-Olarte, Esteban; Moreno, Edgardo (American Society for Microbiology, 2009-04-15)
      Brucella ceti causes disease in Odontoceti. The absence of control serum collections and the diversity of cetaceans have hampered the standardization of serological tests for the diagnosis of cetacean brucellosis. Without ...
    • The differential interaction of brucella and ochrobactrum with innate immunity reveals traits related to the evolution of stealthy pathogens 

      BARQUERO-CALVO, ELIAS; Conde-Álvarez, Raquel; Chacón-Díaz, Carlos; Quesada-Lobo, LucÍa; Martirosyan, Anna; Guzman-Verri, Caterina; Iriarte, Maite; Manček Keber, Mateja; Jerala, Roman; Gorvel, Jean-Pierre; Moriyon, Ignacio; Moreno, Edgardo; Chaves-Olarte, Esteban (PLOS ONE, 2009-06-16)
      Background: During evolution, innate immunity has been tuned to recognize pathogen-associated molecular patterns. However, some a-Proteobacteria are stealthy intracellular pathogens not readily detected by this system. ...
    • Pathology of striped dolphins (Stenella coeruleoalba) infected with Brucella ceti 

      González-Barrientos, Rocio; Morales, Juan Alberto; Hernández-Mora, Gabriela; BARQUERO-CALVO, ELIAS; Guzman-Verri, Caterina; Chaves-Olarte, Esteban; Moreno, Edgardo (Elsevier Ltd, 2009-10-14)
      Seventeen striped dolphins (Stenella coeruleoalba) displaying swimming disorders compatible with neurological syndromes were investigated for Brucella infection. Sixteen dolphins had meningoencephalomyelitis. Serum antibody ...
    • Chromobacterium violaceum infection in a free ranging howler monkey in Costa Rica 

      Baldi, Mario; Morales, Juan Alberto; Hernandez, Giovanna; Jiménez-Soto, Mauricio; Alfaro-Alarcón, Alejandro; BARQUERO-CALVO, ELIAS (Wildlife Disease Association, 2010)
      Chromobacterium violaceum is a gram-negative saprobe bacterium that is a rare opportunistic pathogen in mammals. There are numerous reports in humans including fatalities, but no record exists in free-ranging nonhuman ...
    • The two-component system BvrR/BvrS regulates the expression of the Type IV secretion system VirB in Brucella abortus 

      Martínez-Núñez, Carola; Altamirano-Silva, Pamela; Alvarado-Guillén, Francisco; Moreno, Edgardo; Guzman-Verri, Caterina; Chaves-Olarte, Esteban (American Society for Microbiology, 2010-11)
      The pathogenesis of Brucella is related to the ability to multiply intracellularly, an event controlled by the two-component system BvrR/BvrS (TCS BvrRS) and the type IV secretion machinery VirB (T4SS VirB). We have ...
    • The use of green fluorescent protein as a marker for Brucella vaccines 

      Chacón-Díaz, Carlos; Muñoz, Melissa; BARQUERO-CALVO, ELIAS; Guzman-Verri, Caterina; Chaves-Olarte, Esteban; Grilló, María-Jesús; Moreno, Edgardo (Elsevier, 2010-11-04)
      La brucelosis es una importante enfermedad de los animales de producción y de la fauna silvestre y una zoonosis mundial. El uso de vacunas eficaces y las correspondientes pruebas de diagnóstico que permiten diferenciar los ...