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Trabajos Finales de Graduación

URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/14611

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Examinando Trabajos Finales de Graduación por Autor "Alfaro Vargas, Pamela"

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    Actividad tripanocida de compuestos purificados de plantas del género Hamelia en modelos experimentales in vitro y establecimiento de la infección crónica con Trypanosoma cruzi en un modelo in vivo
    (Universidad Nacional (Costa Rica), 2021) Alfaro Vargas, Pamela; Baizán Rojas, Mónica; Urbina Villalobos, Andrea
    American trypanosomiasis is a prevalent and potentially fatal disease caused by the protozoan Trypanosoma cruzi that represents a serious public health problem in 21 Latin American countries. The available treatments are inefficient and produce severe side effects, which is why there is a need to study new efficient therapies against this disease. In preliminary investigations, an antiparasitic activity of interest was observed in non-purified fractions from Hamelia sp plants, from this, in this work cell infection models were established in vitro and the trypanocidal effect of three compounds extracted from Hamelia sp. . For this, mammalian cell lines were infected with trypomastigotes and the IC50 concentration of three hydroalcoholic compounds (c1-c3) was evaluated by anti-amastigote cytotoxicity assays. Compound c3 was the most potent against amastigotes, and the one with the best selectivity with cells and epimastigotes. The T. cruzi strains used in this study were analyzed according to their discrete typing unit (DTU) by amplifying and sequencing the 18S rDNA region, the phylogenetic tree classified the strains as TcI. In addition, a model of chronic Chagas infection was developed in C57BL / 6 mice that will be essential for the in vivo evaluation of the therapeutic effects of compounds that are selected in the in vitro phase.