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Positivity of Lawsonia intracellularis in seven regions of Costa Rica diagnosed by porcine fecal samples using Real time PCR
(Anatomical Society, 2019-08) Chaverri Esquivel, Laura; Chacón Ureña, Fernando; Alfaro-Alarcon, Alejandro; Zeledón Donzo, Daniel Francisco ; Esquivel, Milena; Guerrero, Roger
Lawsonia intracellularis is an obligate intracellular, motile, curved, gram-negative bacterium that resides freely within the apical cytoplasm of infected intestinal enterocytes (Pusterla & Gebhart, 2013). Europe, Asia and North America have shown an individual prevalence of 24 to 47%. Herd prevalence for finishing pigs in other EU countries has been estimate to range between 88 and 100% (Pascu et al, 2015). Clinical signs: hypoproteinemia/hypoalbuminemia, thickened small intestinal loops on ultrasonographic evaluation and ruling out other causes of enteropathy and protein losses (Pusterla &Gebhart, 2013). The losses due to enteropathy are estimate by at least US $ 1.53 per affected animal (Moller, 2006). In Costa Rica, we reported two cases by histopathological findings: severe chronic active linfo-histio plasmocitic with eosinophils ileo-tiflitis with peyers patches hyperplasia. Real time qPCR confirmed L. intracellularis. The Cts for the samples were 24 and 20 cycles.