Escuela de Ciencias Agrarias
URI permanente para esta comunidadhttp://10.0.96.45:4000/handle/11056/14688
La Escuela de Ciencias Agrarias (ECA) de la Universidad Nacional, genera conocimientos y capacidades por medio de la docencia, investigación, extensión y servicios, que contribuyen al desarrollo de la agricultura sostenible, los agronegocios y la competitividad, y de los espacios rurales y territoriales, para mejorar el desarrollo humano sostenible y equitativo de la sociedad.
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Página web: www.agrarias.una.ac.cr/
Correo: agrarias@una.cr
Teléfono: (506) 2277-3092 / (506) 2277-3296 / (506) 2277-3566
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Ítem Four new species and five morphospecies of Ficophagus Davies & Bartholomaeus, 2015 (Nematoda: Aphelenchoididae) from Ficus subgenus Urostigma sect. Americana (Moraceae) in Central America(Brill & The Hague Academy of International Law, 2017-05-02) Davier, Kerrie A.; Ye, Weimin; Kanzaki, Natsumi; Center, Barbara; Bartholomaesus, Faerlie W.; Herre, Edward Allen; Esquivel, Alejandro; Giblin-Davis, RobinFour new species of Ficophagus were recovered from the sycones of Ficus spp. from the Subgenus Urostigma section Americana from Central America. The species are described here as Ficophagus bullenei sp. n., F. costaricanus sp. n., F. paraensis sp. n. and F. kayai sp. n. The new species are differentiated from other species of the genus by a combination of morphological characters including body length, position of the excretory pore, spicule shape, stylet form, length of the post-uterine sac, tail shape, sequence data of the ribosomal DNA 18S, 28S D2-D3 and mitochondrial DNA cytochrome oxidase subunit I, biogeographical range, and host wasp and Ficus species affiliations. In addition, five morphospecies are described from Ficus cahuitensis, F. citrifolia, F. dugandii, F. obtusifolia, and F. trigonata, respectively. The morphospecies are not formally named due to a lack of pertinent data/molecular sequences.Ítem Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica(Springer, 2016-06-25) Peraza-Padilla, Walter; Archidona-Yuste, Antonio; Cantalapiedra-Navarrete, Carolina; Zamora-Araya, Tatiana; Palomares-Rius, Juan Emilio; Castillo, PabloCorrect identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus. Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees. © 2016, Koninklijke Nederlandse Planteziektenkundige Vereniging.Ítem Molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Costa Rica, with notes on Xiphinema setariae Tarjan, 1964(Springer, 2016-12-16) Peraza-Padilla, Walter; Archidona-Yuste, Antonio; Ferris, Howard; Zamora-Araya, Tatiana; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan Emilio; Subbotin, Sergei; Castillo, PabloPseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.Ítem Two new species and three morphospecies of Ficophagus Davies & Bartholomaeus, 2015 (Nematoda: Aphelenchoididae) from Ficus subgenus Pharmacosycea (Moraceae) in Central America(Brill & The Hague Academy of International Law, 2017-03-15) Davies, Kerrie A; Ye, Weimin; Center, Barbara; Kanzaki, Natsumi; Bartholomaeus, Faerlie; Herre, Edward Allen; Esquivel, Alejandro; Giblin-Davis, RobinAphelenchoidid nematodes were collected from the sycones of Ficus subgenus Pharmacosycea from Central America. Two new species of Ficophagus were recovered, and are described herein as F. maxima sp. n. and F. yoponensis sp. n. from Ficus maxima and F. yoponensis, respectively. They are differentiated from other species of the genus by a combination of morphological characters including having C-shaped females and spiral males, EP opening close to the lips, a short to long post-uterine sac, spicule characters, three pairs of subventral caudal papillae, DNA sequence data, biogeographical range, and host wasp and Ficus species affiliation. The new species are differentiated from each other by spicule characters, length of the post-uterine sac, spermatheca shape, and female tail shape. In addition, three morphospecies were collected from sycones of Ficus glabrata, F. insipida and F. tonduzii, respectively. Their morphological descriptions are presented but these taxa are not formally named as they currently lack molecular data.