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URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/26464

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Direct immunofluorescence test for Campylobacter fetus subsp. venerealis in breeding bulls of Costa Rica
(Universidad Nacional, Costa Rica, 2025-09-30) Navarro, Leonel; Urbina, Andrea; Chacón, Jorge
Bovine genital campylobacteriosis (BGC) is a venereal infectious disease caused by a Gram-negative bacillus Campylobacter fetus subspecies venerealis (Cfv) that impairs the reproductive efficiency in cattle herds. The clinical diagnosis of BGC is made due to low fertility in the breeding herd but must be confirmed by laboratory methods. The distribution of the disease is worldwide, especially in cattle herds where natural mating is the main breeding system. However, data from Costa Rica are lacking. This study aimed to detect the presence of Campylobacter fetus subsp. venerealis (Cfv) in scrapings collected from the preputial mucosa of breeding bulls allocated in the North Huetar and North Pacific regions of Costa Rica using direct immunofluorescence (DIF). No data regarding reproductive problems were available in the 157 participating farms. Scrapings of the preputial mucosa were collected using a plastic rod-type aspirator-scraper introduced into the preputial cavity and stored in formaline phosphate buffered at 1 % and subsequently analyzed by DIF. All the bulls tested yielded negative for the pathogen. The possible causes related to this result are discussed.
Haplotypes of Aedes aegypti and Aedes albopictus larvae detected in 18 districts of Costa Rica
(Universidad Nacional, Costa Rica, 2025-07) Beltre-Nuñez, Edwin; Flores-Nuñez, Sonia; Arguello Vargas, Silvia; Solorzano-Morales, Antony; Dolz, Gaby
There are approximately 3,500 species of mosquitoes distributed on all continents except Antarctica. Some of these species are important since they transmit diseases (Aguirre & Navarro, 2017). Aedes aegypti and Aedes albopictus belong to the Culicidae family of the Diptera order and are biological vectors of more than 26 viruses, including dengue virus, chikungunya virus and Zika virus, impacting public health and posing an economic burden on hospitals, especially in tropical and subtropical regions (Cadavid et al., 2015; McKenzie et al., 2019). Approximately two-fifths of the global population are at risk of contracting dengue fever, and an estimated 500,000 people are affected by hemorrhagic disease annually, with mortality rates exceeding 20% (Guo et al., 2016; Hemme et al., 2010). The chikungunya virus can also present as hemorrhagic disease; it also causes meningoencephalitis and myocarditis (Arredondo et al., 2016), whereas Zika causes severe joint pain, Guillain–Barré syndrome and congenital anomalies such as microcephaly (Plourde & Bloch 2016).
Parasite-associated histopathological lesions in free-ranging birds from Costa Rica
(Universidad Nacional, Costa Rica, 2026-02-26) Carballo-Vargas, Jessica; Romero-Vega, Mario; Alfaro-Alarcon, Alejandro
Bird species can serve as both definitive and intermediate hosts for a wide variety of parasites, some of which are important for veterinary and public health. These parasites can elicit different physiological responses in the host, from completely asymptomatic infections to severe complications that impair the animal's health and lead to death. To further study this aspect, we sampled free-ranging birds, of which 53.41% were parasitized, with a prevalence of 34.09% for Coccidia, 23.86% for Trematoda, 14.77% for Nematoda, 9.09% for Cestoda, and 5.68% for Parasitic cysts (Protozoan), while only 20.45% of birds presented histopathological lesions concurrent with adjacent parasite presence. The inflammation presence and severity are more significant in birds infected with Trematoda.

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