Artículos Científicos

URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/17242

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A comparison of the reproductive ability of Varroa destructor (Mesostigmata:Varroidae) in worker and drone brood of Africanized honey bees (Apis mellifera)
(Springer Science, 2007-09-08) Calderón, Rafael A.; Zamora, Luis G.; Van Veen, Johan W.; Quesada, Mariela V.
Colony infestation by the parasitic mite, Varroa destructor is one of the most serious problems for beekeeping worldwide. In order to reproduce varroa females, enter worker or drone brood shortly before the cell is sealed. To test the hypothesis that, due to the preference of mites to invade drone brood to reproduce, a high proportion of the mite reproduction should occur in drone cells, a comparative study of mite reproductive rate in worker and drone brood of Africanized honey bees (AHB) was done for 370 mites. After determining the number, developmental stage and sex of the offspring in worker cells, the foundress female mite was immediately transferred into an uninfested drone cell. Mite fertility in single infested worker and drone brood cells was 76.5 and 79.3%, respectively. There was no difference between the groups (X 2 = 0.78, P = 0.37). However, one of the most significant differences in mite reproduction was the higher percentage of mites producing viable offspring (cells that contain one live adult male and at least one adult female mite) in drone cells (38.1%) compared to worker cells (13.8%) (X 2 = 55.4, P < 0.01). Furthermore, a high level of immature offspring occurred in worker cells and not in drone cells (X 2 = 69, P < 0.01). Although no differences were found in the percentage of non-reproducing mites, more than 74% (n = 85) of the mites that did not reproduce in worker brood, produced offspring when they were transferred to drone brood.
A scientific note on virgin queen acceptance in stingless bees: evidence for the importance of queen aggression
(Springer Nature, 2010-01) Jarau, Stefan; Van Veen, Johan; Aguilar, Ingrid; Ayasse, Manfred
Stingless bees (Apidae, Meliponini), which are close relatives to honey bees (Apidae, Apini) and share with them a highly eusocial colonial organization (Michener, 2000), are remarkable for their habit of producing virgin queens throughout the year (Engels and Imperatriz-Fonseca, 1990). Most of these queens are not needed for a colony’s survival or division, however, and get executed some time after their emergence (Imperatriz-Fonseca and Zucchi, 1995).
Phytochemical Profiling of Clusia valerioi (Standl.) Floral Resin and Its Correlation with the Chemical Composition of Propolis from Southern Costa Rica
(Universidad Nacional, Costa Rica, 2025-04-16) Segura-Víquez, Andrea; Soto-Fallas, Roy M; Borbón-Alpízar, Henry; Umaña-Rojas, Eduardo; Zamora-Fallas, Gabriel; Sánchez-Chaves, Luis; Fallas-Matamoros, Natalia; Montenegro-Hidalgo, Víctor; Alfaro-Alarcón, Alejandro; Guevara-González, Mariana; Romero-Vega, Luis; Loaiza-Montoya, Randall; Picado-Canales, Natalin; Barrantes-Murillo Erika
Abstract Propolis are resinous byproducts produced by bees, known for their wide range of bioactive properties. The chemical composition of propolis is closely linked to the botanical environment surrounding the hive. Clusia valerioi (Standl.), a plant species endemic to Costa Rica, is commonly found in the country's southern region and produces floral resins that are collected by bees for propolis production. This ecological relationship suggests that chemical traceability between C. valerioi floral resins and propolis produced in nearby apiaries is feasible. In this study, ethanolic extracts from propolis samples collected at eight apiaries, resin loads transported by forager bees, and floral resin samples from C. valerioi at four sites near two of these apiaries were analyzed using high- performance thin-layer chromatography (HPTLC). The analysis revealed three chemical markers—referred to as compounds A, B, and C—that support the traceability of chemical constituents from floral resin to propolis. Compound A was identified as a terpene derivative, while compound C was determined to be a flavonoid derivative (specifically a flavonol or flavanone). Compound B was isolated and identified as a polyprenylated polycyclic benzophenone, and was conclusively characterized as nemorosone based on proton (¹H-NMR) and carbon-13 (¹³C-NMR) nuclear magnetic resonance spectra.
Relationship between chemical composition, botanical origin and antiparasitic activity of Costarican propolis against Trypanosoma cruzi
(Universidad de Antioquia, Colombia, 2022) Barrantes-Murillo, Erika; Alfaro- Alarcon, Alejandro; Fallas-Matamoros, Natalia; Guevara-Gonzalez, Mariana; Montenegro-Hidalgo, Victor; Picado-Canales, Natalin; Sanchez- Chaves, Luis Alejandro; Segura-Víquez, Andrea; Soto-Fallas, Roy Mario; Valdes-Diaz, Sandra; Zamora- Fallas, Luis; Zamora-Rodríguez, Mónica; Loaiza-Montoya, Randall; Umaña-Rojas, Eduardo
Chagas disease affects millions of people throughout Latin America; more than 300,000 new cases and 12,000 deaths are reported yearly. Due the high incidence, social and economic impact, the search for efficient, effective, and safe treatments for this disease has become a priority in the region. Based on previously published studies, it has been determined that some propolis extracts with polyisoprenylated benzophenones (BPI), such as nemorosone, have important trypanocidal activity. Furthermore, BPIs with activity against Trypanosoma cruzi can be found in floral resins of species of the genus Clusia in the southern region of Costa Rica. Therefore, this study proposes to evaluate in vitro and in vivo the antiparasitic effect of propolis containing BPI from the Southern Region of Costa Rica in the search for novel molecules that could be used as a potential treatment for chronic experimental trypanosomiasis. We hypothesize, that propolis is a better source for a wide range of concentrated BPIs than the original botanical source. For this study, 20 samples of propolis from the southern region of Costa Rica were taken from 10 randomly selected apiaries. The samples will be purified using medium pressure chromatographic techniques (MPLC) and the chemical composition will be determined by HPTLC (high-performance thin layer chromatography). At the in vitro level, we will work with the 3T3 cell line to evaluate the cytotoxicity of the compounds. The cytotoxicity and selectivity will be tested in the T. cruzi clone Dm28c. We also propose to validate the safety and efficacy of the selected compounds in vivo in C57B6/J mice.

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Examinando Artículos Científicos por browse.metadata.procedence "Centro de Investigaciones Apícolas Tropicales (CINAT)"