Seguimiento del ciclo estral en Choloepus hoffmanni en cautiverio en Costa Rica, mediante la medición de metabolitos fecales de progesterona y estradiol
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Fecha
2015-09-08
Autores
Herra Vargas, Sofía
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Universidad Nacional, Costa Rica
Resumen
El análisis de esteroides fecales es un método no invasivo que permite conocer el
estado fisiológico del individuo. Actualmente, existe poca información disponible acerca
del comportamiento reproductivo del perezoso de dos dedos, Choloepus hoffmani. Este
estudio se realizó en individuos en cautiverio con el fin de (i) conocer si la extracción de
progesterona y estradiol a partir de heces y su medición por medio del equipo AIA-360®
funcionan como una técnica confiable y (ii) determinar si existe correlación entre las
mediciones hormonales en plasma y las extracciones de muestras fecales.
El muestreo se realizó de noviembre de 2013 a enero de 2014, en el centro de
rescate “Sloth Sanctuary” en Cahuita, Limón, Costa Rica. Se analizaron 269 muestras de
heces y 18 de sangre, provenientes de cinco hembras sexualmente maduras.
Tras la extracción hormonal fecal, se obtuvo un promedio de 217,46 ng/g (IC95%:
132,31 – 302,61 ng/g) de progesterona y 1704,04 pg/g (IC95%: 1597,29-1810,79 pg/g) de
estradiol.
No se observó un efecto del individuo sobre las concentraciones fecales de estradiol
ni progesterona. No se encontró una correlación estadísticamente significativa entre la
progesterona plasmática y la fecal; sin embargo, los datos sugieren que la concentración
hormonal plasmática se ve reflejada en heces hasta los dos días posteriores al evento
sanguíneo. La duración promedio del ciclo estral se estimó en 24,80 días (IC95%: 12,34 ±
37,26 días).
Se pudo demostrar que la técnica propuesta permite la detección exitosa y el
seguimiento de variaciones hormonales, aunque no remplaza las mediciones plasmáticas
para determinar valores absolutos.
The use of fecal steroids measurement is a non-invasive approach that has increased the comprehension of the physiology of several species. Currently, very little is known about the reproductive performance of the two-toed sloth, Choloepus hoffmani. This study was conducted in captive specimens in order to determine (i) the reliability of the fecal progesterone and oestrogen extraction and its quantification with an AIA-360® analyzer, and (ii) if there is a correlation between fecal and plasmatic steroids. The study was performed from November 2013 to January 2014 at the “Sloth Sanctuary” (Cahuita, Limón, Costa Rica). A total of 269 fecal samples and 18 blood samples were collected from five sexually mature females. The average concentration of progesterone was 217.46 ng/g (IC95%: 132.31 – 302.61 ng/g) and of oestradiol was 1704.04 pg/g (IC95%: 1597.29-1810.79 pg/g). No specific individual effect was observed. There was no strong statistical correlation between the fecal and plasmatic progesterone, however the data suggest that the plasmatic events are revealed in the fecal samples obtained two days afterwards. The ovarian cycle was 24.80 days in average (IC95%: 12.34 ± 37.26 days). The results demonstrate that the proposed technique allows the successful detection and follow up of hormonal variations, although it does not replace the plasmatic measurement as a technique to determine absolute values
The use of fecal steroids measurement is a non-invasive approach that has increased the comprehension of the physiology of several species. Currently, very little is known about the reproductive performance of the two-toed sloth, Choloepus hoffmani. This study was conducted in captive specimens in order to determine (i) the reliability of the fecal progesterone and oestrogen extraction and its quantification with an AIA-360® analyzer, and (ii) if there is a correlation between fecal and plasmatic steroids. The study was performed from November 2013 to January 2014 at the “Sloth Sanctuary” (Cahuita, Limón, Costa Rica). A total of 269 fecal samples and 18 blood samples were collected from five sexually mature females. The average concentration of progesterone was 217.46 ng/g (IC95%: 132.31 – 302.61 ng/g) and of oestradiol was 1704.04 pg/g (IC95%: 1597.29-1810.79 pg/g). No specific individual effect was observed. There was no strong statistical correlation between the fecal and plasmatic progesterone, however the data suggest that the plasmatic events are revealed in the fecal samples obtained two days afterwards. The ovarian cycle was 24.80 days in average (IC95%: 12.34 ± 37.26 days). The results demonstrate that the proposed technique allows the successful detection and follow up of hormonal variations, although it does not replace the plasmatic measurement as a technique to determine absolute values
Descripción
Modalidad: Tesis
Palabras clave
PEREZOSO, CICLO ESTRAL, REPRODUCCION ANIMAL, HECES, DIAGNOSTICO DE LABORATORIO, QUIMICA CLINICA, HEMATOLOGIA, HORMONAS, PROGESTERONA, ESTROGENOS, OVARIO, REPRODUCCION SEXUAL