Biotecnologías reproductivas en bovinos, sincronización y transferencia de embriones in vivo realizada en el Instituto de Reproducción Animal Córdoba, Argentina.
Fecha
2015-02-17
Autores
Brenes Jiménez, Cinthya Pamela
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Editor
Universidad Nacional, Costa Rica
Resumen
La pasantía se realizó en el Instituto de Reproducción Animal Córdoba, localizado en la
provincia de Córdoba, Argentina; centro reconocido por su constante aporte en el área de la
biotecnología reproductiva aplicada a bovinos y a la capacitación de médicos veterinarios. Las
técnicas que favorecen el rápido avance en los programas de mejoramiento genético, han
tomado gran importancia debido a la alta demanda y búsqueda de productividad; en la
actualidad, la transferencia de embriones es utilizada en todo el mundo y se transfieren más de
500.000 embriones bovinos por año.
Los programas de superovulación tienen como objetivo principal, inducir el mayor número de
ovulaciones, que resulten en un alto número de embriones transferibles y en aceptables tasas
de preñez luego de la transferencia.
Para los programas de superovulación se utilizaron en total 45 vacas donantes de embriones,
de las razas Brangus, Braford y Aberdeen Angus, obteniéndose en promedio 8,5; 2,0 y 2,7
embriones congelados por animal por raza, respectivamente.
La variabilidad individual es un factor siempre presente en un programa de transferencia de
embriones, ya que existen animales que responden al tratamiento de superestimulación de
manera excelente, regular o mala. En la presente pasantía el 29,2% vacas de la raza Brangus y
66,7% de Braford, presentaron nula respuesta al tratamiento superovulatorio, catalogándose
como vacas problema.
En cuanto a los porcentajes de preñez de las receptoras de embriones congelados se alcanzó
una tasa de aprovechamiento del 79,2% y una tasa de preñez del 47,6%. Finalmente, la aplicación de tratamientos que controlan la dinámica folicular y la ovulación, ofrecen la
ventaja de poder programar los protocolos de superovulación rápidamente, obviando la
detección de celo y realizando la transferencia de embriones a tiempo fijo
The internship was performed in the Instituto de Reproducción Animal Córdoba (Institute of Animal Reproduction Córdoba) localized in the province of Córdoba, Argentina. The institute is recognized for its constant contribution to the reproductive biotechnology field applied in bovines, it is also recognized for training veterinary physicians. The techniques that benefit the quick progress of the genetic enhancement programs have become important due to the high demand and the search for productivity. Currently the embryo transfer is used worldwide and more than 500.000 bovine embryos are transferred every year. The aim of the superovulatory programs is to induce a larger number of ovulations that result in a higher number of transferable embryos and in an acceptable pregnancy rate after the transfer. A total of 45 embryo donor cows were used for the superovulation programs from the Brangus, Braford and Aberdeen-Angus breeds, obtaining an average of 8.5, 2.0 and 2.7 frozen embryos per breed respectively. The individual variability is a factor that is always present in an embryo transfer program since there are animals that respond to the superestimulation treatment in an excellent, regular or bad form. The cows did not respond to the superovulatory treatment were 29.2% for Brangus breed and 66.7% for Braford breed, these breeds were cataloged as “Cattle problem”. The pregnancy rates achieved in the cows that received frozen embryos were: 79.2% of performance ratio and 47.6% of pregnancy rate. Finally, the application of treatments that control follicular dynamics and ovulation, offer the advantage programing superovulation protocols in a faster way, ignoring heat detection and performing fixed-time embryo transfer
The internship was performed in the Instituto de Reproducción Animal Córdoba (Institute of Animal Reproduction Córdoba) localized in the province of Córdoba, Argentina. The institute is recognized for its constant contribution to the reproductive biotechnology field applied in bovines, it is also recognized for training veterinary physicians. The techniques that benefit the quick progress of the genetic enhancement programs have become important due to the high demand and the search for productivity. Currently the embryo transfer is used worldwide and more than 500.000 bovine embryos are transferred every year. The aim of the superovulatory programs is to induce a larger number of ovulations that result in a higher number of transferable embryos and in an acceptable pregnancy rate after the transfer. A total of 45 embryo donor cows were used for the superovulation programs from the Brangus, Braford and Aberdeen-Angus breeds, obtaining an average of 8.5, 2.0 and 2.7 frozen embryos per breed respectively. The individual variability is a factor that is always present in an embryo transfer program since there are animals that respond to the superestimulation treatment in an excellent, regular or bad form. The cows did not respond to the superovulatory treatment were 29.2% for Brangus breed and 66.7% for Braford breed, these breeds were cataloged as “Cattle problem”. The pregnancy rates achieved in the cows that received frozen embryos were: 79.2% of performance ratio and 47.6% of pregnancy rate. Finally, the application of treatments that control follicular dynamics and ovulation, offer the advantage programing superovulation protocols in a faster way, ignoring heat detection and performing fixed-time embryo transfer
Descripción
Modalidad: Pasantía
Palabras clave
BOVINOS, BIOTECNOLOGIA, EMBRION, REPRODUCCION ANIMAL, ARGENTINA