Detección de agentes zoonóticos (Ehrlichia spp., Rickettsia spp., Anaplasma platys y Borrelia burgdorferii s.l.) en garrapatas recolectadas de perros en Costa Rica

Abstract

De octubre 2006 a julio del 2007 se recolectaron 165 garrapatas de perros atendidos en clínicas veterinarias ubicadas en el Valle Central (157) y de algunas residencias (8) en cinco provincias de Costa Rica. En el caso de garrapatas adultas se analizó una garrapata por perro, mientras que en el caso de ninfas se analizaron en grupos de cinco. El ADN extraído de las garrapatas se sometió a diferentes técnicas de PCR anidado para determinar la presencia de especies de Ehrlichia, Rickettsia y Anaplasma platys. En Alemania se analizaron las muestras de garrapatas con un PCR en Tiempo Real para determinar la presencia de B. burgdorferi s.l. Finalmente, una muestra positiva por agente fue secuenciada y comparada con secuencias del GenBank mediante un BlastFrom October 2006 to July 2007, 165 ticks were collected from dogs treated at veterinary clinics in the from dogs treated at veterinary clinics located in the Central Valley (157) and from some (157) and from some residences (8) in five provinces of Costa Rica. Costa Rica. In the case of adult ticks, one tick per dog was analyzed. ticks per dog, while in the case of nymphs, ticks were analyzed in groups of five. nymphs were analyzed in groups of five. The DNA extracted from the ticks DNA extracted from ticks was subjected to different nested PCR techniques to determine the presence of Ehrlichia species. the presence of Ehrlichia, Rickettsia and Anaplasma platys species. platys. In Germany, tick samples were analyzed with a Real Time PCR to determine the presence of Ehrlichia, Rickettsia and Anaplasma platys species. a Real-Time PCR to determine the presence of B. burgdorferi s.l. Finally, one positive specimen per agent was sequenced and compared with sequences from GenBank by a Blast a Blast.