Detección y genotipificación de rotavirus bovino y porcino en Costa Rica
Fecha
2007-10
Autores
Bonilla Espinoza, José Luis
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ISSN de la revista
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Editor
Universidad Nacional, Costa Rica
Resumen
Treinta y ocho muestras fecales de bovinos con diarrea neonatal, positivas
a rotavirus grupo A por inmunocromatografía y electroforesis en gel de
poliacrilamida (PAGE) fueron analizadas mediante reverso transcripción y reacción
en cadena de la polimerasa múltiple anidada/ (nested multiplex/RT-PCR) para la
identificación de genotipos G y P. Las muestras fueron colectadas en 17 fincas
lecheras localizadas en Río Cuarto de Grecia, Pital, Poás, Zarcero, Sucre y
Pacayas.
La genotipificación completa fue posible en 28 (74%) de 38 muestras
fecales. En las muestras de origen bovino, el gen P pudo ser identificado en 17
(61%) muestras; mientras que el gen G fue identificado en 25 (89%) muestras. De
las veintiocho muestras caracterizadas, 10 (40%) correspondieron al genotipo
G10, 8 (32%) a G6 y 7 (28%) a G8, las cuales son cepas comúnmente
encontradas en bovinos; mientras que en lo que respecta a los grupos P, 10 (58%)
muestras correspondieron a P[11], 2 (12%) a P[1], y 1 (6%) a P[7]. Las infecciones
mixtas se presentaron en el grupo P, en 4 (24%) muestras, correspondiendo a
P[11]+P[1] con 2(12%) y P[11]+P[7] con 2(12%) de las muestras. Entre las
combinaciones de los genotipos G y P se obtuvieron, 4 (26%) correspondientes a
G8P[11], 4 (24%) a G6P[11], 3 (19%) a G8P[7], 2 (12%) a G10P[1], 3 (19%) a
G10P[11], y 1 (6%) a G8P[1].
En las muestras de origen porcino 7 muestras fueron positivas por PCR de
muestras colectadas en 5 granjas porcinas en Río Cuarto de Grecia. El gen P
pudo ser identificado en 7 (14%) muestras; mientras que el gen G fue identificado
únicamente en 2 (4%) muestras, los cuales correspondieron al genotipo G5, cepas
comúnmente encontradas en porcinos; mientras que en los grupos P, 4 (57%)
muestras correspondieron a P[6] y 3 (43%) correspondieron a infecciones mixtas
encontrándose 1 muestra perteneciente a P[6]+P[1] y 2 muestras
correspondientes a P[6]+P[7]. Entre las combinaciones de los genotipos G y P se
obtuvieron 2 (28.6%) muestras correspondientes a G5P[6] y G5P[6]+P[1], siendo
esta última una combinación con una infección mixta junto con una cepa inusual
como es P[1].
Esta investigación reporta por primera vez en Centroamérica la
caracterización molecular de cepas de rotavirus asociadas a diarrea neonatal en
terneros y porcinos.
Thirty-eight fecal samples from cattle with neonatal diarrhea, positive for rotavirus group A by immunochromatography and polyacrylamide gel electrophoresis (PAGE) were analyzed by reverse transcription and nested multiplex polymerase chain reaction (nested multiplex/RT-PCR) for the identification of G and P genotypes. Samples were collected from 17 dairy farms located in Río Cuarto de Grecia, Pital, Poás, Zarcero, Sucre and Pacayas. Complete genotyping was possible in 28 (74%) of 38 fecal samples. In the samples of bovine origin, the P gene could be identified in 17 (61%) samples; while the G gene was identified in 25 (89%) samples. Of the twenty-eight samples characterized, 10 (40%) corresponded to the G10 genotype, 8 (32%) to G6 and 7 (28%) to G8, which are strains commonly found in cattle; while regarding the P groups, 10 (58%) samples corresponded to P[11], 2 (12%) to P[1], and 1 (6%) to P[7]. Mixed infections occurred in the P group in 4 (24%) samples, corresponding to P[11]+P[1] with 2(12%) and P[11]+P[7] with 2(12%) of the samples. Among the combinations of G and P genotypes, 4 (26%) corresponded to G8P[11], 4 (24%) to G6P[11], 3 (19%) to G8P[7], 2 (12%) to G10P[1], 3 (19%) to G10P[11], and 1 (6%) to G8P[1]. In the swine samples, 7 samples were positive by PCR from samples collected in 5 pig farms in Rio Cuarto de Grecia. The P gene could be identified in 7 (14%) samples; while the G gene was identified in only 2 (4%) samples, which corresponded to the G5 genotype, strains commonly found in swine; while in the P groups, 4 (57%) samples corresponded to P[6] and 3 (43%) corresponded to mixed infections, with 1 sample belonging to P[6]+P[1] and 2 samples corresponding to P[6]+P[7]. Among the combinations of G and P genotypes, 2 (28.6%) samples corresponding to G5P[6] and G5P[6]+P[1] were obtained, the latter being a combination with a mixed infection together with an unusual strain such as P[1]. This research reports for the first time in Central America the molecular characterization of rotavirus strains associated with neonatal diarrhea in calves and swine.
Thirty-eight fecal samples from cattle with neonatal diarrhea, positive for rotavirus group A by immunochromatography and polyacrylamide gel electrophoresis (PAGE) were analyzed by reverse transcription and nested multiplex polymerase chain reaction (nested multiplex/RT-PCR) for the identification of G and P genotypes. Samples were collected from 17 dairy farms located in Río Cuarto de Grecia, Pital, Poás, Zarcero, Sucre and Pacayas. Complete genotyping was possible in 28 (74%) of 38 fecal samples. In the samples of bovine origin, the P gene could be identified in 17 (61%) samples; while the G gene was identified in 25 (89%) samples. Of the twenty-eight samples characterized, 10 (40%) corresponded to the G10 genotype, 8 (32%) to G6 and 7 (28%) to G8, which are strains commonly found in cattle; while regarding the P groups, 10 (58%) samples corresponded to P[11], 2 (12%) to P[1], and 1 (6%) to P[7]. Mixed infections occurred in the P group in 4 (24%) samples, corresponding to P[11]+P[1] with 2(12%) and P[11]+P[7] with 2(12%) of the samples. Among the combinations of G and P genotypes, 4 (26%) corresponded to G8P[11], 4 (24%) to G6P[11], 3 (19%) to G8P[7], 2 (12%) to G10P[1], 3 (19%) to G10P[11], and 1 (6%) to G8P[1]. In the swine samples, 7 samples were positive by PCR from samples collected in 5 pig farms in Rio Cuarto de Grecia. The P gene could be identified in 7 (14%) samples; while the G gene was identified in only 2 (4%) samples, which corresponded to the G5 genotype, strains commonly found in swine; while in the P groups, 4 (57%) samples corresponded to P[6] and 3 (43%) corresponded to mixed infections, with 1 sample belonging to P[6]+P[1] and 2 samples corresponding to P[6]+P[7]. Among the combinations of G and P genotypes, 2 (28.6%) samples corresponding to G5P[6] and G5P[6]+P[1] were obtained, the latter being a combination with a mixed infection together with an unusual strain such as P[1]. This research reports for the first time in Central America the molecular characterization of rotavirus strains associated with neonatal diarrhea in calves and swine.
Descripción
Maestría en Enfermedades Tropicales
Palabras clave
ENFERMEDADES DE LOS ANIMALES, VIROSIS, DIARREA, DIARRHEA, TERNEROS, CERDO, GENOTIPOS, GENOTYPING, VIRULENCE, PIG, INMUNOLOGÍA VETERINARIA, ENFERMEDADES INFECCIOSAS, INFECTIOUS DISEASES, VETERINARY IMMUNOLOGY