Medroxiprogesterona acetato para la elaboración de dispositivos intravaginales caseros usados en la sincronización del estro en ovinos de pelo: Evaluación de su eficacia, sus efectos secundarios y comparación con un dispositivo comercial en hatos de Costa Rica.
Fecha
2017-06-28
Autores
González, Silvia
Luna-Tortós, Carlos
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Editor
Universidad Nacional, Costa Rica
Resumen
El objetivo del trabajo fue determinar la eficacia farmacológica y los efectos secundarios de un dispositivo intravaginal
de fabricación casera elaborado con acetato de medroxiprogestrona para la sincronización del estro en ovinos de pelo.
Se incluyeron un total de 78 hembras adultas, multíparas y ciclando en un estudio de dos etapas. En la primera etapa
se utilizaron 18 animales con el objetivo de analizar la eficacia y los efectos secundarios del dispositivo de elaboración
casera. En la segunda etapa se utilizaron 60 hembras divididas aleatoriamente en dos grupos: (G1) dispositivo
casero (n-30), (G2) dispositivo comercial (n-30). El fin de esta etapa fue comparar la tasa de sincronización de celos
y la tasa de concepción por monta natural. Para la sincronización se colocó el dispositivo con 60mg de acetato de
medroxiprogesterona durante 12 días, con inyección de 200 UI de eCG y 5 mg de dinoprost trometamina al retirar
del dispositivo. Se evaluaron andrológicamente 13 machos, de los cuales 4 fueron descartados por ser insatisfactorios
y se utilizaron 8 machos satisfactorios durante ambas fases del estudio. En ambas etapas la presentación de celo fue
nula durante la presencia del dispositivo y 100% presentaron celo 48 horas después de su retiro. Los efectos adversos
en la primera etapa fueron: Vaginitis leve al día 12 en todas las hembras, 3 presentaron adherencias, 2 hembras con
aumento de fibrinógeno el día 12 y una disminución general de los leucocitos. En la segunda etapa no hubo diferencias
en la sincronización entre los grupos, la tasa de concepción fue de 80,0% y 76,7% en G1 y G2, respectivamente. Basado
en los resultados de este trabajo, el dispositivo intravaginal de fabricación casera es efectivo para la sincronización del
estro en ovinos de pelo y para la obtención de altas tasas de preñez por monta natural.
The goal of our study was to determine the pharmacological efficacy and adverse effects of a homemade intravaginal device containing medroxyprogesterone acetate for estrus synchronization in hair ewes. A total of 78 adult, multiparous, cycling females were included in the two-phase experiment. During the first phase 18 ewes were included, and the efficacy and adverse effects of the homemade device were evaluated. In the second phase 60 ewes were aleatory assigned into two experimental groups: (G1) homemade intravaginal device (n-30), and (G2) commercial device (n-30) with the goal of comparing the estrus synchronization rate and conception rate among groups when using natural mating. The protocol consisted of 12-days treatment with 60mg of medroxyprogesterone acetate (MAP) by using the intravaginal device, followed by 200 UI eCG and 5 mg dinoprost tromethamine injection on day 12. A total of 13 males were tested by complete andrological evaluation, 8 were included as satisfactory males for the experiments. During both phases no signs of estrus were observed during the treatment with MAP, and 100% showed estrous signs 48 hours after device withdrawal. Adverse effects observed in the first part included: Mild vaginitis in all ewes, 3 females presented adherences, 2 showed fibrinogen increase at day 12, and a general lower leucocyte count after treatment was observed. In the second phase, no differences were observed among groups, and conception rates were 80.0% and 76.7% for G1 and G2 respectively. We conclude that the homemade intravaginal device is as effective as a commercial one for estrus synchronization, which allows obtaining high pregnancy rates in hairy sheep in Costa Rica.
The goal of our study was to determine the pharmacological efficacy and adverse effects of a homemade intravaginal device containing medroxyprogesterone acetate for estrus synchronization in hair ewes. A total of 78 adult, multiparous, cycling females were included in the two-phase experiment. During the first phase 18 ewes were included, and the efficacy and adverse effects of the homemade device were evaluated. In the second phase 60 ewes were aleatory assigned into two experimental groups: (G1) homemade intravaginal device (n-30), and (G2) commercial device (n-30) with the goal of comparing the estrus synchronization rate and conception rate among groups when using natural mating. The protocol consisted of 12-days treatment with 60mg of medroxyprogesterone acetate (MAP) by using the intravaginal device, followed by 200 UI eCG and 5 mg dinoprost tromethamine injection on day 12. A total of 13 males were tested by complete andrological evaluation, 8 were included as satisfactory males for the experiments. During both phases no signs of estrus were observed during the treatment with MAP, and 100% showed estrous signs 48 hours after device withdrawal. Adverse effects observed in the first part included: Mild vaginitis in all ewes, 3 females presented adherences, 2 showed fibrinogen increase at day 12, and a general lower leucocyte count after treatment was observed. In the second phase, no differences were observed among groups, and conception rates were 80.0% and 76.7% for G1 and G2 respectively. We conclude that the homemade intravaginal device is as effective as a commercial one for estrus synchronization, which allows obtaining high pregnancy rates in hairy sheep in Costa Rica.
Descripción
Palabras clave
OVINOS, SHEEP, REPRODUCCION ANIMAL, ANIMAL REPRODUCTION, COSTA RICA