Perfil de toxinas de tipo paralizante de algunas especies de dinoflagelados del Golfo de Nicoya, Puntarenas, Costa Rica
Archivos
Fecha
2011
Autores
Berrocal Artavia, Karen
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Nacional (Costa Rica)
Resumen
Se recolectaron muestras frescas de fitoplancton en diferentes áreas del Golfo de Nicoya con una red de 20 gm de abertura de malla. Se aislaron cepas de los dinoflagelados Gymnodinium catenatum, Cochlodinium Cf. catenatum, Akashiwo sanguinea, Lingulodinium polyedrum, Alexandrium monilatum y Alexandrium fraterculus y se cultivaron en el medio de cultivo Ll y se mantuvieron a una temperatura de 26 ± 1 0C y salinidad de 31 psu, con un fotoperiodo de 12 h luz: 12 h oscuridad y a una intensidad lumínica de aproximadamente 68 pmol fotón m-- s-l . Se determinó la tasa de crecimiento y concentrados celulares para la detección de toxinas de tipo paralizante para cada especie mediante cromatografía líquida con detección flourimétrica (LC-FLD) y derivatización postcolumna. A. sanguinea y G. catenatum presentaron las mayores tasas de crecimiento (0.4 divisiones/día) y A. fratercu/us la más baja (0.1 divisiones/día). Las especies A. monilatum y A. fraterculus produjeron quistes de resistencia y L. polyedrum y A. sanguinea quistes temporales, G. catenatum solo produjo planocigotos y en C. Cf catenatum no se observó ningún tipo de reproducción sexual. L. polyedrum y G. catenatum presentaron un ciclo sexual heterotálico y A. monilatum y A. fraterculus homotálico. G. catenatum fue la única especie que presentó toxinas de tipo paralizante: Cl, C2, GTXs l, 2, 3 y 4 y dcGTXs 2 y 3. Siendo GTX 2 la toxina que aportó más del 70% y dcGTX 3 la que se encontró en menor cantidad (O. 13%).
Fresh phytoplankton samples were collected in different areas of the Gulf of Nicoya with a 20 gm mesh size net. Strains of the dinoflagellates Gymnodinium catenatum, Cochlodinium Cf. catenatum, Akashiwo sanguinea, Lingulodinium polyedrum, Alexandrium monilatum and Alexandrium fraterculus were isolated and cultivated in the culture medium Ll and kept at a temperature of 26 ± 1 0C and salinity of 31 psu. , with a photoperiod of 12 h light: 12 h dark and at a light intensity of approximately 68 pmol photon m-- s-l . The growth rate and cell concentrates for the detection of paralytic-type toxins were determined for each species by liquid chromatography with fluorimetric detection (LC-FLD) and post-column derivatization. A. sanguinea and G. catenatum had the highest growth rates (0.4 divisions/day) and A. fratercu/us the lowest (0.1 divisions/day). The species A. monilatum and A. fraterculus produced resistance cysts and L. polyedrum and A. sanguinea temporary cysts, G. catenatum only produced planozygotes and in C. Cf catenatum no type of sexual reproduction was observed. L. polyedrum and G. catenatum presented a heterothallic sexual cycle and A. monilatum and A. fraterculus homothallic. G. catenatum was the only species that presented paralytic type toxins: Cl, C2, GTXs 1, 2, 3 and 4 and dcGTXs 2 and 3. GTX 2 being the toxin that contributed more than 70% and dcGTX 3 the one that was found. in smaller quantity (0. 13%).
Fresh phytoplankton samples were collected in different areas of the Gulf of Nicoya with a 20 gm mesh size net. Strains of the dinoflagellates Gymnodinium catenatum, Cochlodinium Cf. catenatum, Akashiwo sanguinea, Lingulodinium polyedrum, Alexandrium monilatum and Alexandrium fraterculus were isolated and cultivated in the culture medium Ll and kept at a temperature of 26 ± 1 0C and salinity of 31 psu. , with a photoperiod of 12 h light: 12 h dark and at a light intensity of approximately 68 pmol photon m-- s-l . The growth rate and cell concentrates for the detection of paralytic-type toxins were determined for each species by liquid chromatography with fluorimetric detection (LC-FLD) and post-column derivatization. A. sanguinea and G. catenatum had the highest growth rates (0.4 divisions/day) and A. fratercu/us the lowest (0.1 divisions/day). The species A. monilatum and A. fraterculus produced resistance cysts and L. polyedrum and A. sanguinea temporary cysts, G. catenatum only produced planozygotes and in C. Cf catenatum no type of sexual reproduction was observed. L. polyedrum and G. catenatum presented a heterothallic sexual cycle and A. monilatum and A. fraterculus homothallic. G. catenatum was the only species that presented paralytic type toxins: Cl, C2, GTXs 1, 2, 3 and 4 and dcGTXs 2 and 3. GTX 2 being the toxin that contributed more than 70% and dcGTX 3 the one that was found. in smaller quantity (0. 13%).
Descripción
Berrocal Artavia, K. (2011). Perfil de toxinas de tipo paralizante de algunas especies de dinoflagelados del Golfo de Nicoya, Puntarenas, Costa Rica. [Tesis de Licenciatura]. Universidad Nacional, Heredia, C.R.
Palabras clave
FITOPLANCTON, TOXINAS, TOXINS, MICROBIOLOGY, MICROBIOLOGIA, BIOLOGIA, GOLFO DE NICOYA (COSTA RICA), PUNTARENAS (COSTA RICA)