Molecular techniques in the characterization of Leishmania isolates from Central America
Fecha
1995
Autores
Carreira, P.F.
Maingon, R.
Ward, R. D.
Noyes, H
Ponce, C.
Belli, A
Arana, B.
Zeledón, Rodrigo
Sousa, O. E.
Título de la revista
ISSN de la revista
Título del volumen
Editor
Taylor & Francis
Resumen
Los problemas de salud pública causados por la leishmaniasis en la mayoría de los países de Centroamérica se están volviendo cada vez más más grave. Esto se debe en parte al tamaño cada vez mayor de las poblaciones humanas que están en riesgo y a su patrones migratorios. Incidencia anual de la enfermedad en Costa Rica, Honduras, Guatemala, Panamá y Se estima que en Nicaragua hay hasta 20.000 casos. Los cambios regionales en la epidemiología de los distintos Leishmania spp. Los presentes han enfatizado la necesidad de herramientas de diagnóstico innovadoras, sensibles y precisas. PCR e isoenzima, anticuerpo monoclonal, esquizodema, sonda de ADN y amplificación aleatoria, polimórfica Se han realizado análisis de ADN. Indicios preliminares de que Leishmania chagasi estaba presente en Costa Rica y Honduras y que se produjeron híbridos interespecíficos en Nicaragua se han confirmado utilizando estos métodos. También se encontró que la distribución del complejo mexicana era más amplia y heterogénea. de lo esperado inicialmente. En general, hubo una concordancia del 87% entre los resultados obtenidos utilizando el diferentes técnicas.
The public-health problems caused by leishmaniasis in most countries in Central America are becoming more severe. This is partly because of the increasing size of the human populations that are at risk and their migratory patterns. Annual incidence of the disease in Costa Rica, Honduras, Guatemala, Panama and Nicaragua is estimated to be as high as 20 000 cases. Regional changes in the epidemiology of the various Leishmania spp. present have emphasized the need for innovative, sensitive and accurate diagnostic tools. PCR and isoenzyme, monoclonal antibody, schizodeme, DNA-probe and random-amplified, polymorphic DNA analyses have been tested. Preliminary indications that Leishmania chagasi was present in Costa Rica and Honduras and that interspecific hybrids occurred in Nicaragua have been confirmed using these methods. The distribution of the mexicana complex was also found to be broader and more heterogeneous than initially expected. Overall, there was 87o/o concordance between the results produced using the different techniques.
The public-health problems caused by leishmaniasis in most countries in Central America are becoming more severe. This is partly because of the increasing size of the human populations that are at risk and their migratory patterns. Annual incidence of the disease in Costa Rica, Honduras, Guatemala, Panama and Nicaragua is estimated to be as high as 20 000 cases. Regional changes in the epidemiology of the various Leishmania spp. present have emphasized the need for innovative, sensitive and accurate diagnostic tools. PCR and isoenzyme, monoclonal antibody, schizodeme, DNA-probe and random-amplified, polymorphic DNA analyses have been tested. Preliminary indications that Leishmania chagasi was present in Costa Rica and Honduras and that interspecific hybrids occurred in Nicaragua have been confirmed using these methods. The distribution of the mexicana complex was also found to be broader and more heterogeneous than initially expected. Overall, there was 87o/o concordance between the results produced using the different techniques.
Descripción
Palabras clave
ENFERMEDAD DE CHAGAS, COSTA RICA, ZOONOSIS, LEISHMANIASIS, AMÉRICA CENTRAL, CHAGAS DISEASE, CENTRAL AMERICA