Determinación in vitro de la potencia del extracto de pituitaria del pargo mancha (Lutjanus guttatus) (Steindachner, 1869)
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Fecha
2004
Autores
López Garro, Andrés M
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Universidad Nacional (Costa Rica)
Resumen
El pargo mancha (L. guttatus), es una especie de gran importancia en las pesquerías de la costa Pacífica de Costa Rica. Debido al descenso en la producción pesquera y al apetecido valor de su carne en el mercado, surge la necesidad de reproducir esta especie en cautiverio por parte del sector acuícola nacional.
Para esto se deben controlar diferentes etapas del ciclo de vida, tales como reproducción, crecimiento, nutrición, etc Para el caso de L. guttatus su reproducción ha sido posible mediante la inducción hormonal con compuestos sintéticos como la HCG, OVAPRIM, etc. Además, se utilizan extractos de la pituitaria de algunos peces como la carpa (SPC).
En esta investigación se determinó la potencia del extracto de pituitaria del pargo mancha (SPPM 9 y $ ) en UI (Unidades Internacionales), mediante la técnica in vitro. Para ello se aplicaron diferentes tratamientos o concentraciones de HCG y SSM (® y 3) a oocitos maduros, midiendo el incremento en diámetro y madurez a las 24 y 48 horas (IDP24-48 y IMP24-48) de exposición.
The spotted snapper (L. guttatus) is a species of great importance in the fisheries of the Pacific coast of Costa Rica. Due to the decrease in fishing production and the desired value of its meat in the market, the need arises to reproduce this species in captivity by the national aquaculture sector. For this, different stages of the life cycle must be controlled, such as reproduction, growth, nutrition, etc. In the case of L. guttatus, its reproduction has been possible through hormonal induction with synthetic compounds such as HCG, OVAPRIM, etc. In addition, extracts from the pituitary of some fish such as carp (SPC) are used. In this investigation, the potency of the pituitary extract of the spotted snapper (SPPM 9 y $ ) in IU (International Units) was determined, using the in vitro technique. For this, different treatments or concentrations of HCG and SSM (® and 3) were applied to mature oocytes, measuring the increase in diameter and maturity at 24 and 48 hours (IDP24-48 and IMP24-48) of exposure.
The spotted snapper (L. guttatus) is a species of great importance in the fisheries of the Pacific coast of Costa Rica. Due to the decrease in fishing production and the desired value of its meat in the market, the need arises to reproduce this species in captivity by the national aquaculture sector. For this, different stages of the life cycle must be controlled, such as reproduction, growth, nutrition, etc. In the case of L. guttatus, its reproduction has been possible through hormonal induction with synthetic compounds such as HCG, OVAPRIM, etc. In addition, extracts from the pituitary of some fish such as carp (SPC) are used. In this investigation, the potency of the pituitary extract of the spotted snapper (SPPM 9 y $ ) in IU (International Units) was determined, using the in vitro technique. For this, different treatments or concentrations of HCG and SSM (® and 3) were applied to mature oocytes, measuring the increase in diameter and maturity at 24 and 48 hours (IDP24-48 and IMP24-48) of exposure.
Descripción
López Garro, A. M. (2004). Determinación in vitro de la potencia del extracto de pituitaria del pargo mancha (Lutjanus guttatus) (Steindachner, 1869). [Tesis de Licenciatura]. Universidad Nacional, Costa Rica.
Palabras clave
PARGO, PARGO CON MANCHA, REPRODUCCION ANIMAL, ANIMAL REPRODUCTION, SPOTTED SNAPPER