Concentración y tiempo máximo de exposición de juveniles de pargo manchado Lutjanus guttatusal eugenol Syzygium aromaticum
Fecha
2019-01
Autores
Chacón Guzmán, Jonathan
Carvajal Oses, Milagro
Herrera-Ulloa, Angel
Pauletto, Silvia
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Editor
Universidad Nacional (Costa Rica)
Resumen
Se definieron las dosis más eficientes de aceite de eugenol Syzygium aromaticum para la sedación quirúrgica en juveniles de pargo manchado (Lutjanus guttatus) y se determinó el tiempo mínimo de exposición de no retorno, como una fuente de información para contar con un rango de tiempo seguro en la manipulación del pez durante labores de rutina. En el Laboratorio de Acuicultura y Biotecnología Marina del Parque Marino del Pacífico en el Golfo de Nicoya, se seleccionaron 76 juveniles de pargo manchado, producidos en enero del 2016, con un peso promedio 21.61± 2.0 g. Los juveniles fueron expuestos a concentraciones de eugenol 25, 100, 175 y 250 mg/L en tanques de 30 L, se vigiló los estados de inducción a la anestesia y su recuperación. Definida la dosis más eficiente, se procedió con ella a determinar el tiempo máximo de exposición; para este estudio se definieron cuatro tiempos de inducción en 4, 8, 12 y 16 min., y se establecieron 10 min., como umbral de recuperación. Se determinó que el eugenol es eficaz para la anestesia de juveniles de pargo con temperaturas entre 24.0 y 24.8°C. La concentración de 25 mg/L fue suficiente para la inducción parcial de los peces, apropiado para realizar actividades rutinarias menos complejas en el manejo de pargos, mientras que la concentración más eficiente fue de 100 mg/L, en tiempos de exposición no mayor a 8 minutos luego de la anestesia total.
The most efficient doses of eugenol oil from Syzygium aromaticum for surgical sedation in Lutjanus guttatus juveniles were defined, and the minimum exposure time of no return was determined as a source of information to have a safe time range when handling fish during routine work. A total of 76 juveniles produced in January 2016 with an average weight of 21.61 ± 2.0 g were selected at the Aquaculture and Marine Biotechnology Laboratory of the Marino del Pacífico Park (Gulf of Nicoya). Juveniles were exposed to 25, 100, 175, and 250 mg/L eugenol concentrations in 30 L tanks, and their induction of anesthesia and recovery were monitored. Once the most efficient dose was defined, the maximum exposure time was determined. Four times of induction were defined for this study: 4, 8, 12, and 16 min, and the recovery threshold was established at 10 min. Eugenol was determined to be an effective anesthetic for rose snapper juveniles with temperatures ranging between 24.0 and 24.8°C. The 25 mg/L concentration was sufficient for partial induction of fish and appropriate to perform less complex routine activities in the handling of snappers, while the most efficient concentration was 100 mg/L, with exposure times no greater than 8 minutes after total anesthesia.
The most efficient doses of eugenol oil from Syzygium aromaticum for surgical sedation in Lutjanus guttatus juveniles were defined, and the minimum exposure time of no return was determined as a source of information to have a safe time range when handling fish during routine work. A total of 76 juveniles produced in January 2016 with an average weight of 21.61 ± 2.0 g were selected at the Aquaculture and Marine Biotechnology Laboratory of the Marino del Pacífico Park (Gulf of Nicoya). Juveniles were exposed to 25, 100, 175, and 250 mg/L eugenol concentrations in 30 L tanks, and their induction of anesthesia and recovery were monitored. Once the most efficient dose was defined, the maximum exposure time was determined. Four times of induction were defined for this study: 4, 8, 12, and 16 min, and the recovery threshold was established at 10 min. Eugenol was determined to be an effective anesthetic for rose snapper juveniles with temperatures ranging between 24.0 and 24.8°C. The 25 mg/L concentration was sufficient for partial induction of fish and appropriate to perform less complex routine activities in the handling of snappers, while the most efficient concentration was 100 mg/L, with exposure times no greater than 8 minutes after total anesthesia.
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Palabras clave
PARGO, ACUICULTURA, LUTJANUS GUTTATUS, AQUACULTURE, COSTA RICA