Efecto del AIA y el AIB sobre el enraizamiento in vitro de brotesde Sechium edule (Jacq.) Sw
Fecha
2015-01
Autores
García García, José
Salas Alvarado, Eduardo
Azofeifa Bolaños, José
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Instituto de Biotecnología de las Plantas
Resumen
La presente investigación tuvo como finalidad determinar la respuesta de brotes de chayote [Sechium edule(Jacq.) Sw.] a la aplicación de ácido indol butírico (AIB) y ácido-3-indol acético (AIA) en la fase de enraizamiento.Se estudió el efecto de tres medios de cultivo, modificados a partir del propuesto por Murashige y Skoog(MS): 1- Sales MS al 65%, 2- Sales MS al 65% + 0.05 mg l-1 de ácido indolbutírico (AIB), y 3- Sales MS al65% + 3.0 mg l-1 de ácido-3 indolácetico (AIA). Las variables evaluadas fueron: número de raíces, longitud ynúmero de brotes por plántula in vitro. El mejor resultado se logró al utilizar el medio de cultivo MS al 65%con 3.0 mg l-1 de AIA. Se encontraron diferencias significativas (P<0.05), entre tratamientos que conteníanlos reguladores de crecimiento, para las variables número de raíces y longitud de plántulas in vitro. En laaccesión UNA-730 la inducción de raíces se produjo en un medio de cultivo con sales MS al 65% sinreguladores de crecimiento; sin embargo, cuando se adicionó AIA o AIB la inducción fue del 100%. Lasdiferencias indicadas en la inducción de raíces en el presente trabajo probablemente son el resultado conjuntodel genotipo y las condiciones específicas de cultivo
This investigation was carried out with the objective of evaluating the response of shoots of chayote [S. edule (Jacq.) Sw.] to the application of indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) on the in vitro rooting phase. The effect of three culture media, modified from the proposed of Murashige and Skoog (MS) was studied: 1- 65% MS salt base, 2- 65% MS salt base + 0.05 mg l-1 of indolebutyric acid (AIB) and 3- 65% MS salt base + 3.0 mg l-1-3-indole acetic acid (IAA). The variables evaluated were: number of roots, length and number of shoots per in vitro seedling. The best result were obtained when using 65% MS salt base with 3.0 mg l-1 of IAA. Significant differences (P<0.05) among treatments containing growth regulators was obtained for the variables number of roots and length of in vitro plantlets. In the UNA-730 accession root induction was obtained in a 65% MS salt base culture medium without growth regulators; however, when IAA and IBA was added the induction was 100%. The differences identified in this work with regards to the root induction were probably the combined result of genotype and specific culture conditions.
This investigation was carried out with the objective of evaluating the response of shoots of chayote [S. edule (Jacq.) Sw.] to the application of indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) on the in vitro rooting phase. The effect of three culture media, modified from the proposed of Murashige and Skoog (MS) was studied: 1- 65% MS salt base, 2- 65% MS salt base + 0.05 mg l-1 of indolebutyric acid (AIB) and 3- 65% MS salt base + 3.0 mg l-1-3-indole acetic acid (IAA). The variables evaluated were: number of roots, length and number of shoots per in vitro seedling. The best result were obtained when using 65% MS salt base with 3.0 mg l-1 of IAA. Significant differences (P<0.05) among treatments containing growth regulators was obtained for the variables number of roots and length of in vitro plantlets. In the UNA-730 accession root induction was obtained in a 65% MS salt base culture medium without growth regulators; however, when IAA and IBA was added the induction was 100%. The differences identified in this work with regards to the root induction were probably the combined result of genotype and specific culture conditions.
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CHAYOTE, CULTIVO IN VITRO, FISIOLOGÍA VEGETAL, HORTALIZAS, PRODUCTOS AGROPECUARIOS, IN VITRO CULTIVATION, PLANT PHYSIOLOGY, AGRICULTURAL PRODUCTS, VEGETABLES