Multiplicación masiva de genotipos seleccionados de Tectona grandis en medio semisólido y en Sistemas de Inmersión Temporal, como una herramienta para programas de mejoramiento genético
Fecha
2021
Autores
Jiménez Corrales, Lucía
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Resumen
La teca (Tectona grandis Linn) es una especie forestal reconocida como madera preciosa,
de alto valor económico. Se propaga exitosamente por propagación vegetativa y por semilla,
no obstante, estos procesos son lentos, por lo que se requiere optimizar técnicas de
micropropagación que minimicen el tiempo de producción. Tanto los Sistemas de Inmersión
Temporal (SIT) como los medios semisólidos (MSS), con la adición de reguladores de
crecimiento como el 6–Bencilaninopurina (BAP) producen multiplicación de teca. En la
presente investigación se evaluaron SIT y MSS, así como concentraciones de 0,0; 0,5; 1,0 y
1,5 mg/L de BAP para la multiplicación de dos genotipos de plantas seleccionadas de teca
como parte de un programa de mejoramiento genético. Los resultados obtenidos mostraron
que ambos sistemas son eficientes para multiplicar teca, con superioridad en los MSS, sin
embargo, es importante determinar un adecuado balance de los sistemas y las citoquinas para
evitar la hiperhidricidad de las plantas. Los coeficientes de multiplicación obtenidos para el
genotipo 618 fueron de 6,55 y 5,35, para MSS y SIT respectivamente, mientras que para el
genotipo 117 se obtuvieron coeficientes de multiplicación de 12,20 y 9,35, para MSS y SIT
respectivamente.
Teak (Tectona grandis Linn) is a forest species recognized as precious wood, of high economic value. It is propagated successfully by vegetative propagation and by seed, However, these processes are slow, so it mis necessary to optimize techniques of micropropagation that minimize production time. Both the Immersion SystemsTemporal (SIT) as the semi-solid media (MSS), with the addition of buffers growth such as 6-benzylaninopurine (BAP) produce theca multiplication. In the In this investigation, SIT and MSS were evaluated, as well as concentrations of 0.0; 0.5; 1.0 and 1.5 mg/L of BAP for the multiplication of two genotypes of selected teak plants as part of a genetic improvement program. The results obtained showed that both systems are efficient to multiply teak, with superiority in MSS, without However, it is important to determine an adequate balance of systems and cytokines to avoid hyperhydricity of plants. The multiplication coefficients obtained for the genotype 618 were 6.55 and 5.35, for MSS and SIT respectively, while for the genotype 117, multiplication coefficients of 12.20 and 9.35 were obtained for MSS and SITrespectively.
Teak (Tectona grandis Linn) is a forest species recognized as precious wood, of high economic value. It is propagated successfully by vegetative propagation and by seed, However, these processes are slow, so it mis necessary to optimize techniques of micropropagation that minimize production time. Both the Immersion SystemsTemporal (SIT) as the semi-solid media (MSS), with the addition of buffers growth such as 6-benzylaninopurine (BAP) produce theca multiplication. In the In this investigation, SIT and MSS were evaluated, as well as concentrations of 0.0; 0.5; 1.0 and 1.5 mg/L of BAP for the multiplication of two genotypes of selected teak plants as part of a genetic improvement program. The results obtained showed that both systems are efficient to multiply teak, with superiority in MSS, without However, it is important to determine an adequate balance of systems and cytokines to avoid hyperhydricity of plants. The multiplication coefficients obtained for the genotype 618 were 6.55 and 5.35, for MSS and SIT respectively, while for the genotype 117, multiplication coefficients of 12.20 and 9.35 were obtained for MSS and SITrespectively.
Descripción
Jiménez Corrales, L. (2021). Multiplicación masiva de genotipos seleccionados de Tectona grandis en medio semisólido y en Sistemas de Inmersión Temporal, como una herramienta para programas de mejoramiento genético. [Tesis de Licenciatura]. Universidad Nacional, Heredia, C.R.
Palabras clave
TECA, TECTONA GRANDIS, CULTIVO IN VITRO, PLANTAS, BIOTECNOLOGÍA VEGETAL, FLOORS, VEGETAL BIOTECHNOLOGY