New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis
Date
2011-06-11Author
López-Goñi, Ignacio
García-Yoldi, David
Marín Alcalá, Clara María
de Miguel, María J.
BARQUERO-CALVO, ELIAS
Guzman-Verri, Caterina
Albert, David
Garin-Bastuji, Bruno
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Show full item recordAbstract
Rapid and specific identification of Brucella suis at the biovar level is necessary because
some of the biovars that infect animals are pathogenic for humans. None of the molecular
typing methods described so far are able to discriminate B. suis biovars in a single test and
differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This
article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate
identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and
Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows
the correct discrimination of all known Brucella species is also described. La identificación rápida y específica de Brucella suis a nivel de biovariedades es necesaria porque algunas de las biovariedades que infectan a los animales son patógenas para los seres humanos. Ninguno de los métodos de tipificación molecular descritos hasta ahora es capaz de discriminar las biovariedades de B. suis en una sola prueba y la diferenciación de B. suis de Brucella canis mediante enfoques moleculares puede ser difícil. Este artículo describe un nuevo ensayo de PCR multiplex, Suis-ladder, para la identificación rápida y precisa de B. suis a nivel de biovariedad y la diferenciación de B. suis, B. canis y Brucella microti. También se describe un avance del protocolo de PCR Bruce-ladder original que permite la discriminación correcta de todas las especies conocidas de Brucella.
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