Logotipo del repositorio

Artículos Científicos

URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/14690

Examinar

Examinando Artículos Científicos por Autor "Archidona-Yuste, Antonio"

Seleccione resultados tecleando las primeras letras
Mostrando 1 - 2 de 2
  • Miniatura
    Ítem
    Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica
    (Springer, 2016-06-25) Peraza-Padilla, Walter; Archidona-Yuste, Antonio; Cantalapiedra-Navarrete, Carolina; Zamora-Araya, Tatiana; Palomares-Rius, Juan Emilio; Castillo, Pablo
    Correct identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus. Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees. © 2016, Koninklijke Nederlandse Planteziektenkundige Vereniging.
  • Miniatura
    Ítem
    Molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Costa Rica, with notes on Xiphinema setariae Tarjan, 1964
    (Springer, 2016-12-16) Peraza-Padilla, Walter; Archidona-Yuste, Antonio; Ferris, Howard; Zamora-Araya, Tatiana; Cantalapiedra-Navarrete, Carolina; Palomares-Rius, Juan Emilio; Subbotin, Sergei; Castillo, Pablo
    Pseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.