Artículos Científicos
URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/14690
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Examinando Artículos Científicos por Autor "Araya-Valverde, Emanuel"
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Ítem SNP Markers Found in Non‑coding Regions can Distinguish Among Low‑variant Genotypes of Arabica and other Cofee Species(Springer Nature, 2022-12-10) Bolívar‑González, Alejandro; Molina-Bravo, Ramón; Solano-Sánchez, William; Araya-Valverde, Emanuel; Ivamoto-Suzuki, Suzana T.; Pereira, Luiz F. P.; Gatica-Arias, AndrésDevelopment of efcient and scalable methods for molecular identifcation of Cofea spp. are necessary to accelerate studies related to the characterization of germplasm for both conservation or breeding purposes, and the validation of cofee germplasm. The low genetic diversity of cofee hinders the establishment of protocols that facilitate the molecular characterization of a given genotype. In this study, nucleotide variability was analyzed at 22 loci in the genome of 19 cofee accessions using de novo primer sets and high-resolution melting (HRM). Single nucleotide polymorphisms (SNPs) variants were studied in coding regions of genes implicated in sucrose accumulation in the seed, Sucrose synthase 2 (SUS2), Ent kaurene oxidase 1 (CaKO1), and Cafeoyl-coenzyme A 3-O-methyltransferase (CcOAOMT). The non-coding Internal transcribed spacer 2 (ITS2) region was also studied. Variability was shown at 103 positions both at the interspecies level (15 loci) and among varieties of Cofea arabica L. (4 loci). The HMR technique for identifcation of variants in genes CaKO1, SUS2, CcoAOMT, as well as in the ITS2 region proved to be a robust technique for germplasm characterization. More important this technique can be used for fngerprinting and traceability of cofee grain exports which is an increasing market-consumer demand.