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Influence of Physico-Chemical Factors on the Efficiency and Metabolite Profile of Adult Pinus radiata D. Don Bud Organogenesis

dc.contributor.authorRojas Vargas, Alejandra
dc.contributor.authorCastander-Olarieta , Ander
dc.contributor.authorMontalbán, Itziar Aurora
dc.contributor.authorMoncalean, Paloma
dc.date.accessioned2023-09-28T18:10:32Z
dc.date.available2023-09-28T18:10:32Z
dc.date.issued2022-09-10
dc.descriptionFinanciado por el Departamento de Junta de Becas de la Universidad Nacional de Costa Rica y por el proyectos: MINECO (AGL2016-76143-C4-3R), MICINN (PID2020-112627RB-C32), CYTED (P117RT0522), MULTIFOREVER project, under the umbrella of ERA-NET Cofund ForestValue by ANR (FR), FNR (DE), MINCyT (AR), MINECO-AEI (ES), MMM (FI), VINNOVA (SE). ForestValue has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement no. 773324 y al DECO (Basque government).
dc.description.abstractGenetic improvement programs for conifer forest species face the challenge of propagating elite individuals with superior characteristics in the present landscape of climate change; the problem is focused on the fact that when these individuals have shown the desirable traits, they have changed phase and therefore have lost the ability to be propagated by traditional methods. Based on our previous works on Pinus spp. regeneration of adult trees through organogenesis and trying to improve the protocol in Pinus radiata, our objective was to analyze the influence of collection dates and different 6-benzyladenine (BA) concentrations in the first phase of shoot induction, as well as the effect of different light types on the success of root induction. Moreover, we were interested in studying the effect of the abovementioned physico-chemical factors on the amino acid and carbohydrate content in the shoots developed in vitro. Reinvigorated shoots were obtained in both BA concentrations (22 or 44 M), although the highest BA concentration showed the best results in terms of shoot induction (explants forming shoots (46%) and number of shoots per explant (1.95 0.52)) when using initial explants collected in the first week of February. The percentage of explants forming shoots (EFS) was genotype-dependent. Explants from genotype A induced with the highest BA concentration showed the highest EFS (91%). With respect to the light treatment applied, significant differences in root induction (20%) and in the number of roots per explant (4.62 0.65) were observed in shoots cultured under white FL. Finally, significant differences in different phases of the rooting process were detected in the amounts of fructose, glucose and sucrose and in the content of threonine and tyrosine.es_ES
dc.description.abstractGenetic improvement programs for conifer forest species face the challenge of propagating elite individuals with superior characteristics in the present landscape of climate change; the problem is focused on the fact that when these individuals have shown the desirable traits, they have changed phase and therefore have lost the ability to be propagated by traditional methods. Based on our previous works on Pinus spp. regeneration of adult trees through organogenesis and trying to improve the protocol in Pinus radiata, our objective was to analyze the influence of collection dates and different 6-benzyladenine (BA) concentrations in the first phase of shoot induction, as well as the effect of different light types on the success of root induction. Moreover, we were interested in studying the effect of the abovementioned physico-chemical factors on the amino acid and carbohydrate content in the shoots developed in vitro. Reinvigorated shoots were obtained in both BA concentrations (22 or 44 M), although the highest BA concentration showed the best results in terms of shoot induction (explants forming shoots (46%) and number of shoots per explant (1.95 0.52)) when using initial explants collected in the first week of February. El porcentaje de explantes que formaron brotes (EFS) dependió del genotipo. Los explantes del genotipo A inducidos con la mayor concentración de BA mostraron el mayor EFS (91%). Con respecto al tratamiento lumínico aplicado, se observaron diferencias significativas en la inducción de raíces (20%) y en el número de raíces por explante (4,62 0,65). cultivados bajo FL blanca. Por último, se detectaron diferencias significativas en las distintas fases del proceso de enraizamiento detectadas en las cantidades de fructosa, glucosa y sacarosa y en el contenido de treonina y tirosies_ES
dc.description.procedenceInstituto de Investigación y Servicios Forestales (INISEFOR)es_ES
dc.description.procedenceNEIKER-BRTA, Department of Forestry Scienceses_ES
dc.description.sponsorshipUniversidad Nacional, Costa Ricaes_ES
dc.identifier.issn1999-4907
dc.identifier.urihttp://hdl.handle.net/11056/26510
dc.language.isoenges_ES
dc.publisherUniversidad Nacional, Costa Ricaes_ES
dc.rightsAcceso abiertoes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.sourceForests 2022, 13, 1455es_ES
dc.subjectLEDses_ES
dc.subjectMICROPROPAGATIONes_ES
dc.subjectMICROPROPAGACIÓNes_ES
dc.subjectRADIATA PINEes_ES
dc.subjectPINO RADIATAes_ES
dc.subjectROOTINGes_ES
dc.subjectENRAIZAMIENTOes_ES
dc.subjectSHOOT INDUCTIONes_ES
dc.subjectINDUCCIÓN DE BROTESes_ES
dc.titleInfluence of Physico-Chemical Factors on the Efficiency and Metabolite Profile of Adult Pinus radiata D. Don Bud Organogenesises_ES
dc.typehttp://purl.org/coar/resource_type/c_6501es_ES

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