Establecimiento in vitro de Cryptomeria japonica (Taxocidaceae)
Fecha
2003
Autores
Hine, Ana
Valverde Cerdas, Lisette
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Editor
Universidad de Costa Rica
Resumen
En este trabajo se logró establecer brotes in vitro de Cryptomeria japonica a partir de árboles de 20 años. Los brotes fueron desinfectados y tratados con seis concentraciones diferentes de kinetina y belciladenina para inducir su desarrollo y brotación. Se evaluó el efecto de la calidad y la intensidad de la iluminación sobre estos utilizando para ello luz naranja a 20 μ Es-1m-2 y luz blanca a 30 μ Es-1m-2. Para el enraizamiento de los brotes se utilizaron diferentes concentraciones de NAA (ácido naftaleno acético) e IBA (ácido indolbutírico) solos o combinados. El BA y el KIN indujeron la formación de brotes en Cryptomeri, pero se observó la mejor brotación con BA a 9,1μ M. La luz blanca y la luz naranja promovieron el crecimiento de los explantes, así como el crecimiento de nuevos brotes, pero fue mayor con la luz naranja. Se observó el enraizamiento de las yemas, pero no fue posible encontrar la mejor concentración de auxina para el enraizamiento debido a la abundante formación de callos en la base de los explantes y la formación de raíces fue muy esporádica. Los brotes enraizados se colocaron en un sustrato para su aclimatación en condiciones de invernadero.
In this work, it was achieved to stablish in vitro shoots of Cryptomeria japonicafrom 20 year oldtrees. The shoots were disinfected and treated with six different concentrations of kinetin and belciladenine inorder to induce their development and budding. It was evaluated the effect of quality and lighting intensity onthese using for this orange light at 20 μ Es-1m-2and white light at 30 μ Es-1m-2. For shoots rooting it was used dif-ferent concentrations of NAA (naftalen acetic acid) and IBA (indolbutiric acid) alone or combined. BA and KINinduced the bud formation in Cryptomeriabut it was observed the best budding with BA at 9.1μ M. White lightand orange light promoved the growth of explants as well as the growth of new buds but it was higher withorange light. The bud rooting was observed but it was not posible to find the best auxin concentration for root-ing because of the plentiful callus formation on the base of explants and the root formation was very sporadic. The rooted shoots were placed on a substrate for their acclimatation in greenhouse conditions.
In this work, it was achieved to stablish in vitro shoots of Cryptomeria japonicafrom 20 year oldtrees. The shoots were disinfected and treated with six different concentrations of kinetin and belciladenine inorder to induce their development and budding. It was evaluated the effect of quality and lighting intensity onthese using for this orange light at 20 μ Es-1m-2and white light at 30 μ Es-1m-2. For shoots rooting it was used dif-ferent concentrations of NAA (naftalen acetic acid) and IBA (indolbutiric acid) alone or combined. BA and KINinduced the bud formation in Cryptomeriabut it was observed the best budding with BA at 9.1μ M. White lightand orange light promoved the growth of explants as well as the growth of new buds but it was higher withorange light. The bud rooting was observed but it was not posible to find the best auxin concentration for root-ing because of the plentiful callus formation on the base of explants and the root formation was very sporadic. The rooted shoots were placed on a substrate for their acclimatation in greenhouse conditions.
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Palabras clave
CONIFERAS, CULTIVO DE TEJIDOS, FITOMEJORAMIENTO, TÉCNICAS DE CULTIVO, TISSUE CULTIVATION, PLANT BREEDING, CULTIVATION TECHNIQUES