New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis
Abstract
Rapid and specific identification of Brucella suis at the biovar level is necessary because
some of the biovars that infect animals are pathogenic for humans. None of the molecular
typing methods described so far are able to discriminate B. suis biovars in a single test and
differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This
article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate
identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and
Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows
the correct discrimination of all known Brucella species is also described.
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