New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis
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Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described.
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New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis López-Goñi, Ignacio; García-Yoldi, David; Marín Alcalá, Clara María; de Miguel, María J.; BARQUERO-CALVO, ELIAS; Guzman-Verri, Caterina; Albert, David; Garin-Bastuji, Bruno (Veterinary Microbiology, 2011-06-11)Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are ...
Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease Hernández-Mora, Gabriela; Ruiz-Villalobos, Nazareth; Bonilla-Montoya, Roberto; Romero-Zúñiga, Juan José; Jiménez-Arias, Julio; González-Barrientos, Rocio; BARQUERO-CALVO, ELIAS; Chacón-Díaz, Carlos; Rojas, Norman; Chaves-Olarte, Esteban; Guzman-Verri, Caterina; Moreno, Edgardo (PLOS ONE, 2017-08-10)Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR ...
Brucella abortus senses the intracellular environment through the BvrR/Bvrs two-component system, which allows B. abortus to adapt to its replicative nich Altamirano-Silva, Pamela; Meza-Torres, Jazmín; Castillo-Zeledón, Amanda; Ruiz-Villalobos, Nazareth; Zúñiga Pereira, Ana Mariel; Chacón-Díaz, Carlos; Moreno, Edgardo; Guzman-Verri, Caterina; Chaves-Olarte, Esteban (American Society for Microbiology., 2018-01-16)Brucella abortus is a facultative extracellular-intracellular pathogen belonging to a group of Alphaproteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in a membrane-bound ...