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URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/20514
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Examinando Preprints por Materia "ANTIBIOTIC"
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Ítem Optimizing cephaeline and emetine production from Ipecac root and antimicrobial activity through maturity harvesting and processing(2024-09-03) Rodríguez-Víquez, Marco Antonio; Syedd León, Randall Fabio; Rodríguez Rodríguez, Jorengeth Abad; Rodríguez-Rodríguez, Gerardo; Jiménez-Bonilla, Pablo; Álvarez Valverde, Víctor[Objetive] This study aimed to determine whether there is an optimal harvest time for emetine, cephaeline, and total alkaloids Ipecac (Carapichea ipecacuanha [Brot.] L. Andersson) production. Also, the relationship between antibiotic activity and harvest time was tested. is an herb with medicinal properties cultivated in Northern Costa Rica. The root of this plant is valued according to its alkaloid concentration, which underlies its numerous biological activities. [Methodology] Two Ipecac fields from northern Costa Rican producers were sampled every ~2 months during a year. Samples were dried (through sun and oven), ground, extracted, and analyzed by HPLC and titration to evaluate the alkaloid concentration. Also, antimicrobial activity was determined using the Kirbi-Bauer test. [Results] Our main findings revealed that there is no significant difference between the oven-dried samples and the sun-dried samples. Also, an increase in total alkaloid production is observed in roots until during the first 16 months. Also, there is a variation in alkaloid composition: the cephaeline/emetine ratio increases after 16 months. [Conclusions] The highest alkaloid concentration occurs when plants are harvested between 16 and 19 months old. Additionally, the antibiotic activity is maximum at 16 months, and the cephaeline/emetine ratio is 2 at the same harvest time.