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URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/27255
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Examinando Libros por browse.metadata.rights "Attribution-NonCommercial-NoDerivatives 4.0 Internacional"
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Ítem Agua, naturaleza y seguridad hídrica(Universidad Nacional, Costa Rica, 2022) Suárez Serrano, Andrea; Alfaro-Chinchilla, Carolina; Bautista-Solís, PávelBoletín técnico sobre Soluciones Basadas en la Naturaleza (SbN). Los ecosistemas naturales generan importantes servicios ecosistémicos que son utilizados por los ecosistemas naturales y la sociedad. El agua es un recurso estratégico y vital para la vida. En esta obra se revisa el concepto y algunos ejemplos de Soluciones Basadas en la Naturaleza que son importantes para fortalecer la seguridad hídrica de Costa Rica.Ítem Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example(Springer (Alemania), 2018) Zhang, Zhong-Tian; Jiménez-Bonilla, Pablo; Seo, Seung-Oh; Lu, Ting; Jin, Yong-Su; Blaschek, Hans P.; Wang, YiAbstract. CRISPR-Cas9 has been explored as a transformative genome engineering tool for many eukaryotic organisms. However, its utilization in bacteria remains limited and ineffective. This chapter, taking Clostridium beijerinckii as an example, describes the use of Streptococcus pyogenes CRISPR-Cas9 system guided by the single chimeric guide RNA (gRNA) for diverse genome-editing purposes, including chromosomal gene deletion, integration, single nucleotide modification, as well as “clean” mutant selection. The general principle is to use CRISPR-Cas9 as an efficient selection tool for the edited mutant (whose CRISPR-Cas9 target site has been disrupted through a homologous recombination event and thus can survive selection) against? the wild type background cells. This protocol is broadly applicable to other microorganisms for genome-editing purposes.Ítem Biorefinery by the hand of the nanotechnology: biodegradable polymers from industrial biomass waste(ACCEFYN & NanoCiTec (Colombia), 2016) Jose-Roberto, Vega-Baudrit; Hernandez-Miranda, Michael; González-Paz, Rodolfo; Corrales, YendryAbstract. Biorefineries contribute to solve energy, water and environmental problems due to the used of thousands of tons of agricultural biomass residues for production of high value materials, that from a social point of view could help developing countries to improve their economy. The technological and scientific advances in sciences as nanotechnology have increased the understanding of material properties; helping to find new applications. Examples of materials extracted from Costa Rica biomass waste are presented.