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Artículos científicos

URI permanente para esta colecciónhttp://10.0.96.45:4000/handle/11056/14837

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Examinando Artículos científicos por browse.metadata.procedence "Escuela de Medicina Veterinaria"

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    New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis
    (Revista de Microbiología Veterinaria, 2011-06-28) López-Goñi, Ignacio; García-Yoldi, David
    Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described.
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    Parasites of cetaceans stranded on the Pacific coast of Costa Rica
    (Revista de parasitología veterinaria, 2011-05-12) Oliveira, J.B.; Morales, J.A.; González, R.C.; Hernández, J; Hernández, G
    Information regarding parasitic fauna of cetaceans from Costa Rica is provided for the first time. A total of 25 stranded dolphins and whales were examined between 2001 and 2009, including striped dolphin (Stenella coeruleoalba) (n = 19), pantropical spotted dolphin (S. attenuata) (n = 2), spinner dolphin (S. longirostris) (n = 1), bottlenose dolphin (Tursiops truncatus) (n = 1), dwarf sperm whale (Kogia sima) (n = 1) and Cuvier’s beaked whale (Ziphius cavirostris) (n = 1). Pathological findings associated with the parasites are also presented. In the most representative dolphin species, S. coeruleoalba, the prevalence of parasites was 89.5%; moreover, all examined specimens of S. attenuata, S. longirostris, T. truncatus and Z. cavirostris presented parasites. No parasites were recovered from K. sima. Fourteen helminth taxa were identified, including six species of cestodes (Strobilocephalus triangularis, Tetrabothrius forsteri, Trigonocotyle sp., Phyllobothrium delphini, Monorygma grimaldi, Tetraphyllidea gen. sp. plerocercoid), four digeneans (Nasitrema globicephalae, Brachycladium palliatum, B. pacificum and Oschmarinella albamarina) and four nematodes (Anisakis spp., Halocercus lagenorhynchi, Halocercus sp. and Crassicauda anthonyi). A commensal crustacean, Xenobalanus globicipitis, was also identified. All identified parasites representing new geographic records for the Pacific coast of Central America and new host records are presented. Parasitological information is valuable for conservation of cetaceans in Pacific coast of Costa Rica.