Examinando por Autor "Gandaras, Rosario"

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Preparation and Characterization of a Novel Nanocellulose‑Derivative as a Potential Radiopharmaceutical Agent
(Springer (Alemania), 2022) Lecot, Nicole; Gandaras, Rosario; Batista‑Menezes, Diego; Montes de Oca-Vásquez, Gabriela; Cabral, Pablo; García, Ma. Fernanda; Jose-Roberto, Vega-Baudrit; Cerecetto, Hugo; Lopretti, Mary
Abstract. Nanocellulose (NC) has a wide variety of emerging applications, including enzyme immobilization, drug delivery, and imaging diagnosis. On the other hand, derivatives of hydrazinonicotinic acid (HYNIC) have been used as coordination-agents for their binding to 99mTc in the development of potential radiopharmaceuticals. To this end, we studied and developed NC-HYNIC-99mTc for diagnostic imaging using NC obtained from rice husk using Trichoderma reseii and Phanaerochaete chrysosporium in a semi-solid fermentation system to generate a potential nanoradiopharmaceutical agent. In this work, we performed the separation of nanosilica, microcellulose, and nanocellulose using the TAPPI T203 os-74 technique. The synthesis of conjugate NC-HYNIC was performed following a one-pot procedure. The NC and the conjugate NC-HYNIC were characterized by Fourier Transform Infrared Spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and atomic force microscopy (AFM). We obtained NC with a structure of laminar-like nanofibers. The yield of NC was 55% and the conjugate NC-HYNIC was obtained with a yield of 36%. The TGA and FTIR analyses showed that the NC functionalized with HYNIC had similar characteristics to those of NC. In addition, the AFM analysis of the functionalized NC showed an average height of 8 ± 3 nm, while the NC showed an average height of 10 ± 4 nm. The subsequent binding to 99mTc was assayed, and the purity of the radiolabeled product and the efficiency of the process was studied by ITLC chromatography. The radiolabeling process was very efficient with a radiochemical purity of 98 ± 1.2%, which opens the possibility of a new potential-imaging agent.